Rabbit Ubiquitin Polyclonal Antibody | anti-UBB antibody

Anti-Ubiquitin (pS65) Phospho Antibody

Cat. Num. AAA8304199

• Gene Names: UBB; HEL-S-50
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by immunogen affinity chromatography.
• Synonyms: Ubiquitin; Polyclonal Antibody; Anti-Ubiquitin (pS65) Phospho Antibody; UBB; Polyubiquitin-B; UBC; Polyubiquitin-C; RPS27A; UBA80; UBCEP1; Ubiquitin-40S ribosomal protein S27a; Ubiquitin carboxyl extension protein 80; UBA52; UBCEP2; Ubiquitin-60S ribosomal protein L40; CEP52; Ubiquitin A-52 residue ribosomal protein fusion product 1; anti-UBB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of Ubiquitin (pS65) protein.
• Purity/Purification: Purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
• Sequence Length: 229

• Applicable Applications for anti-UBB antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Ubiquitin.
• Preparation and Storage: Upon delivery aliquot and store at-20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of Ubiquitin (pS65) expression in C6 (A), AML12 (B), SGC7901-UV treated (C), SGC7901 (D) whole cell lysates.)

Related Product Information for anti-UBB antibody

Rabbit polyclonal antibody to Ubiquitin (pS65)

NCBI and Uniprot Product Information

• NCBI GI #: 528524471
• NCBI GeneID: 7314
• NCBI Accession #: NP_001268645.1
• NCBI GenBank Nucleotide #: NM_001281716.1
• UniProt Accession #: P0CG47; P02248; P02249; P02250; P62988; Q29120; Q6LBL4; Q6LDU5; Q8WYN8; Q91887; Q91888; Q9BWD6
• Molecular Weight: 25,762 Da
• NCBI Official Full Name: polyubiquitin-B
• NCBI Official Synonym Full Names: ubiquitin B
• NCBI Official Symbol: UBB
• NCBI Official Synonym Symbols: HEL-S-50
• NCBI Protein Information: polyubiquitin-B
• UniProt Protein Name: Polyubiquitin-B
• Protein Family: Ubiquitin
• UniProt Gene Name: UBB

NCBI Description

This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.