RAB27A sirna

RAB27A siRNA (Human)

Cat. Num. AAA829955

• Gene Names: RAB27A; GS2; RAM; RAB27; HsT18676
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAB27A; RAB27A siRNA (Human); RAB27; Ras-related protein Rab-27A; Rab-27; GTP-binding protein Ram; RAB27A sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RAB27A siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 221

• Applicable Applications for RAB27A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RAB27A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAB27A sirna

siRNA to inhibit RAB27A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19923264
• NCBI GeneID: 5873
• NCBI Accession #: NP_004571.2
• NCBI GenBank Nucleotide #: NM_004580.4
• UniProt Accession #: P51159; O00195; Q6FI40; Q9UIR9; Q9Y5U3
• Molecular Weight: 24,041 Da
• NCBI Official Full Name: ras-related protein Rab-27A
• NCBI Official Synonym Full Names: RAB27A, member RAS oncogene family
• NCBI Official Symbol: RAB27A
• NCBI Official Synonym Symbols: GS2; RAM; RAB27; HsT18676
• NCBI Protein Information: ras-related protein Rab-27A
• UniProt Protein Name: Ras-related protein Rab-27A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB27A
• UniProt Synonym Gene Names: RAB27; Rab-27
• UniProt Entry Name: RB27A_HUMAN

NCBI Description

The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

RAB27A: Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Defects in RAB27A are a cause of Griscelli syndrome type 2 (GS2). Griscelli syndrome is a rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 15q15-q21.1

Cellular Component: Golgi apparatus; photoreceptor outer segment; secretory granule membrane; lysosome; late endosome; apical plasma membrane; dendrite; melanosome; secretory granule

Molecular Function: GTPase activity; protein domain specific binding; protein binding; GDP binding; GTP binding; myosin V binding

Biological Process: exocytosis; natural killer cell degranulation; melanosome localization; cytotoxic T cell degranulation; synaptic vesicle transport; protein secretion; melanosome transport; antigen processing and presentation; intracellular protein transport; cellular protein metabolic process; positive regulation of phagocytosis; melanocyte differentiation; Rab protein signal transduction; blood coagulation; vesicle docking during exocytosis; positive regulation of exocytosis; protein targeting

Disease: Griscelli Syndrome, Type 2