Western Blot (WB)
(Western Blot analysis of K-562 cells using RAB27A Monoclonal Antibody at dilution of 1:1000)
Mouse anti-Human RAB27A Monoclonal Antibody | anti-RAB27A antibody
RAB27A Monoclonal Antibody
Gene Names
RAB27A; GS2; RAM; RAB27; HsT18676
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Protein G purification
Synonyms
RAB27A; Monoclonal Antibody; RAB27A Monoclonal Antibody; GS2; GTP-binding protein Ram; HsT18676; MGC117246; Mutant Ras related protein Rab-27A; Rab-27; RAB-27A; RAB27; RAB27A member RAS oncogene family; RAM; Ras-related protein Rab-27A; Ras-related protein Rab27A; RB27A; anti-RAB27A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
200
Purity/Purification
Protein G purification
Concentration
1mg/mL (varies by lot)
Sequence Length
221
Applicable Applications for anti-RAB27A antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
IHC: 1:20-1:200
IF: 1:50-1:500
Immunogen
Fusion protein of RAB27A
Buffer
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of K-562 cells using RAB27A Monoclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of K-562 cells using RAB27A Monoclonal Antibody at dilution of 1:1000)
Related Product Information for anti-RAB27A antibody
Rab27a is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27a is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27a is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.
Product Categories/Family for anti-RAB27A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 25kDa
Observed: 27kDa
Observed: 27kDa
NCBI Official Full Name
ras-related protein Rab-27A
NCBI Official Synonym Full Names
RAB27A, member RAS oncogene family
NCBI Official Symbol
RAB27A
NCBI Official Synonym Symbols
GS2; RAM; RAB27; HsT18676
NCBI Protein Information
ras-related protein Rab-27A
UniProt Protein Name
Ras-related protein Rab-27A
Protein Family
UniProt Gene Name
RAB27A
UniProt Synonym Gene Names
RAB27; Rab-27
NCBI Description
The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.
Research Articles on RAB27A
Similar Products
Product Notes
The RAB27A rab27a (Catalog #AAA2542042) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAB27A Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAB27A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:50-1:500. Researchers should empirically determine the suitability of the RAB27A rab27a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB27A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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