NOTCH1 sirna

NOTCH1 siRNA (Mouse)

Cat. Num. AAA829918

• Gene Names: Notch1; N1; Mis6; Tan1; lin-12; 9930111A19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NOTCH1; NOTCH1 siRNA (Mouse); MOTCH; Neurogenic locus notch homolog protein 1; Notch 1; Motch A; mT14; p300; NOTCH1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: NOTCH1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2531

• Applicable Applications for NOTCH1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse NOTCH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NOTCH1 sirna

siRNA to inhibit NOTCH1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 224967065
• NCBI GeneID: 18128
• NCBI Accession #: NP_032740.3
• NCBI GenBank Nucleotide #: NM_008714.3
• UniProt Accession #: Q01705; Q06007; Q3TZW2; Q3U3Y2; Q61905; Q7TQ50; Q7TQ51; Q7TQ52; Q8K428; Q99JC2; Q9QW58; Q9R0X7
• Molecular Weight: 269,180 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 1
• NCBI Official Synonym Full Names: notch 1
• NCBI Official Symbol: Notch1
• NCBI Official Synonym Symbols: N1; Mis6; Tan1; lin-12; 9930111A19Rik
• NCBI Protein Information: neurogenic locus notch homolog protein 1
• UniProt Protein Name: Neurogenic locus notch homolog protein 1
• Protein Family: Neurogenic locus notch homolog protein
• UniProt Gene Name: Notch1
• UniProt Synonym Gene Names: Motch; Notch 1; NEXT; NICD
• UniProt Entry Name: NOTC1_MOUSE

Uniprot Description

Notch 1: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation. In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. Belongs to the NOTCH family.

Protein type: Receptor, misc.; Transcription factor; Oncoprotein; Membrane protein, integral; Motility/polarity/chemotaxis

Cellular Component: Golgi apparatus; cell surface; membrane; cell; integral to plasma membrane; cytoplasm; acrosome; plasma membrane; integral to membrane; nucleus; receptor complex

Molecular Function: enzyme inhibitor activity; protein binding; enzyme binding; sequence-specific DNA binding; chromatin DNA binding; metal ion binding; receptor activity; calcium ion binding; chromatin binding; transcription factor activity

Biological Process: neural tube development; negative regulation of calcium ion-dependent exocytosis; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; multicellular organismal development; heart development; positive regulation of JAK-STAT cascade; positive regulation of glial cell differentiation; regulation of cell migration; negative regulation of BMP signaling pathway; neuron differentiation; compartment specification; positive regulation of endothelial cell differentiation; negative regulation of ossification; somatic stem cell division; negative regulation of osteoblast differentiation; embryonic limb morphogenesis; positive regulation of cardiac muscle cell proliferation; positive regulation of keratinocyte differentiation; positive regulation of astrocyte differentiation; negative regulation of photoreceptor cell differentiation; positive regulation of neuroblast proliferation; cell fate commitment; transcription, DNA-dependent; neuron fate commitment; keratinocyte differentiation; regulation of transcription from RNA polymerase II promoter; negative regulation of neuron differentiation; branching morphogenesis of a tube; response to muramyl dipeptide; regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; determination of left/right symmetry; negative regulation of transcription, DNA-dependent; anagen; positive regulation of epithelial cell proliferation; foregut morphogenesis; osteoblast fate commitment; endoderm development; transcription from RNA polymerase II promoter; epidermis development; cell fate specification; cardiac muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; regulation of epithelial cell proliferation; negative regulation of neurogenesis; negative regulation of cell proliferation; glial cell differentiation; regulation of neurogenesis; regulation of transcription, DNA-dependent; astrocyte differentiation; cardiac muscle morphogensis; forebrain development; positive regulation of cell proliferation; heart looping; angiogenesis; regulation of somitogenesis; cell differentiation; positive regulation of BMP signaling pathway; regulation of developmental process; mesenchymal cell development; Notch signaling pathway; hair follicle morphogenesis; in utero embryonic development; lumen formation; liver development; humoral immune response; regulation of cell proliferation; activation of Notch receptor target transcription factor; negative regulation of oligodendrocyte differentiation; negative regulation of cell differentiation; inflammatory response to antigenic stimulus; axonogenesis; negative regulation of catalytic activity; epithelial to mesenchymal transition; spermatogenesis; sprouting angiogenesis; auditory receptor cell fate commitment; negative regulation of myoblast differentiation; positive regulation of cell migration; lung development