HAUS1 sirna

HAUS1 siRNA (Human)

Cat. Num. AAA829312

• Gene Names: HAUS1; HEIC; CCDC5; HEI-C; HsT1461
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: HAUS1; HAUS1 siRNA (Human); CCDC5; HEIC; HAUS augmin-like complex subunit 1; Coiled-coil domain-containing protein 5; Enhancer of invasion-cluster; HEI-C; HAUS1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: HAUS1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 278

• Applicable Applications for HAUS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human HAUS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for HAUS1 sirna

siRNA to inhibit HAUS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 19923971
• NCBI GeneID: 115106
• NCBI Accession #: NP_612452.1
• NCBI GenBank Nucleotide #: NM_138443.3
• UniProt Accession #: Q96CS2; Q8N837; B2RDM7
• Molecular Weight: 22,744 Da
• NCBI Official Full Name: HAUS augmin-like complex subunit 1
• NCBI Official Synonym Full Names: HAUS augmin-like complex, subunit 1
• NCBI Official Symbol: HAUS1
• NCBI Official Synonym Symbols: HEIC; CCDC5; HEI-C; HsT1461
• NCBI Protein Information: HAUS augmin-like complex subunit 1
• UniProt Protein Name: HAUS augmin-like complex subunit 1
• Protein Family: HAUS augmin-like complex
• UniProt Gene Name: HAUS1
• UniProt Synonym Gene Names: CCDC5; HEIC; HEI-C
• UniProt Entry Name: HAUS1_HUMAN

NCBI Description

HAUS1 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM, Jun 2010]

Uniprot Description

CCDC5: Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Belongs to the HAUS1 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: spindle pole; microtubule; centrosome; cytoplasm

Molecular Function: protein binding

Biological Process: mitosis; centrosome organization and biogenesis; cell division; spindle assembly