LZTS1 sirna

LZTS1 siRNA (Rat)

Cat. Num. AAA829292

• Gene Names: Lzts1; Psdzip70
• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LZTS1; LZTS1 siRNA (Rat); Leucine zipper putative tumor suppressor 1; PSD-Zip70; LZTS1 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: LZTS1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 601

• Applicable Applications for LZTS1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat LZTS1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LZTS1 sirna

siRNA to inhibit LZTS1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 23618893
• NCBI GeneID: 266711
• NCBI Accession #: NP_703200.1
• NCBI GenBank Nucleotide #: NM_153470.1
• UniProt Accession #: Q8CFC9
• Molecular Weight: 67,565 Da
• NCBI Official Full Name: leucine zipper putative tumor suppressor 1
• NCBI Official Synonym Full Names: leucine zipper, putative tumor suppressor 1
• NCBI Official Symbol: Lzts1
• NCBI Official Synonym Symbols: Psdzip70
• NCBI Protein Information: leucine zipper putative tumor suppressor 1
• UniProt Protein Name: Leucine zipper putative tumor suppressor 1
• Protein Family: Leucine zipper putative tumor suppressor
• UniProt Gene Name: Lzts1
• UniProt Entry Name: LZTS1_RAT

NCBI Description

transcription factor; may play a role cell growth; may be involved with the function of postsynaptic site of excitatory synapses [RGD, Feb 2006]

Uniprot Description

LZTS1: Involved in the regulation of cell growth. May stabilize the active CDC2-cyclin B1 complex and thereby contribute to the regulation of the cell cycle and the prevention of uncontrolled cell proliferation. May act as a tumor suppressor. Defects in LZTS1 may be a cause of esophageal cancer (ESCR). A malignancy of the esophagus. The most common types are esophageal squamous cell carcinoma and adenocarcinoma. Cancer of the esophagus remains a devastating disease because it is usually not detected until it has progressed to an advanced incurable stage. Belongs to the LZTS family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Tumor suppressor

Cellular Component: postsynaptic membrane; neuron projection; postsynaptic density; apical plasma membrane; cytoplasm; dendritic spine; synapse; dendritic shaft; cell junction

Molecular Function: protein binding

Biological Process: negative regulation of macroautophagy; regulation of dendrite morphogenesis; regulation of synaptic plasticity