Mouse HIF1 alpha ELISA Kit | HIF1A elisa kit

Mouse HIF1 alpha ELISA Kit

Cat. Num. AAA8291411

• Gene Names: Hif1a; MOP1; bHLHe78; AA959795; HIF1alpha; HIF1-alpha; HIF-1-alpha
• Reactivity: Mouse
• Synonyms: HIF1 alpha; Mouse HIF1 alpha ELISA Kit; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8; HIF1A elisa kit

• Reactivity: Mouse
• Specificity: The Mouse HIF1 alpha ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse HIF1 alpha proteins within the range of 125 pg/ml - 8000 pg/ml.
• Sequence Length: 837

• Samples: Cell culture supernates, serum, plasma, tissue homogenates
• Sensitivity: < 60 pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HIF1A elisa kit

Principle of the Assay: The MyBioSource' Mouse HIF1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse HIF1 alpha in Cell Culture Supernates, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Mouse HIF1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and HIF1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse HIF1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HIF1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background/Introduction: The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) is the regulated member of the transcription factor heterodimer HIF-1 alpha/beta. HIF-1 alpha binds to hypoxia-response elements (HREs) in the promoters of many genes involved in the adaptation to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

NCBI and Uniprot Product Information

• NCBI GI #: 927028881
• NCBI GeneID: 15251
• NCBI Accession #: NP_001300848.1
• NCBI GenBank Nucleotide #: NM_001313919.1
• UniProt Accession #: Q61221; O08741; O08993; Q61664; Q61665; Q8C681; Q8CC19; Q8CCB6; Q8R385; Q9CYA8
• Molecular Weight: 91,874 Da
• NCBI Official Full Name: hypoxia-inducible factor 1-alpha isoform 1
• NCBI Official Synonym Full Names: hypoxia inducible factor 1, alpha subunit
• NCBI Official Symbol: Hif1a
• NCBI Official Synonym Symbols: MOP1; bHLHe78; AA959795; HIF1alpha; HIF1-alpha; HIF-1-alpha
• NCBI Protein Information: hypoxia-inducible factor 1-alpha
• UniProt Protein Name: Hypoxia-inducible factor 1-alpha
• Protein Family: Hypoxia-inducible factor
• UniProt Gene Name: Hif1a
• UniProt Synonym Gene Names: HIF-1-alpha; HIF1-alpha

NCBI Description

This gene encodes the alpha subunit which, along with the beta subunit, forms a heterodimeric transcription factor that regulates the cellular and developmental response to reduced oxygen tension. The transcription factor has been shown to regulate genes involved in several biological processes, including erythropoiesis and angiogenesis which aid in increased delivery of oxygen to hypoxic regions. The transcription factor also plays a role in the induction of genes involved in cell proliferation and survival, energy metabolism, apoptosis, and glucose and iron metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia ().