Western Blot (WB)
(Western blot analysis of PAGE3 expression in HeLa (A), Raw264.7 (B), H9C2 (C) whole cell lysates.)
Rabbit PAGE3 Polyclonal Antibody | anti-PAGE3 antibody
Anti-PAGE3 Antibody
Gene Names
PAGE3; CT16.6; GAGED1; PAGE-3
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
PAGE3; Polyclonal Antibody; Anti-PAGE3 Antibody; GAGED1; P antigen family member 3; PAGE-3; G antigen family D member 1; Prostate-associated gene 3 protein; anti-PAGE3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of PAGE3 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
113
Applicable Applications for anti-PAGE3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PAGE3. The exact sequence is proprietary.
Preparation and Storage
Upon receipt, aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of PAGE3 expression in HeLa (A), Raw264.7 (B), H9C2 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of PAGE3 expression in HeLa (A), Raw264.7 (B), H9C2 (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PAGE3 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of PAGE3 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-PAGE3 antibody
Rabbit polyclonal antibody to PAGE3
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
P antigen family member 3
NCBI Official Synonym Full Names
P antigen family, member 3 (prostate associated)
NCBI Official Symbol
PAGE3
NCBI Official Synonym Symbols
CT16.6; GAGED1; PAGE-3
NCBI Protein Information
P antigen family member 3
UniProt Protein Name
P antigen family member 3
Protein Family
UniProt Gene Name
PAGE3
UniProt Synonym Gene Names
GAGED1; PAGE-3
UniProt Entry Name
PAGE3_HUMAN
NCBI Description
This gene is a member of family of proteins that are expressed in a variety of tumors and in some fetal and reproductive tissues. Multiple alternatively spliced transcript variants have been observed. [provided by RefSeq, Jan 2015]
Uniprot Description
PAGE3: Belongs to the GAGE family.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: Xp11.21
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Product Notes
The PAGE3 page3 (Catalog #AAA828918) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PAGE3 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's PAGE3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the PAGE3 page3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PAGE3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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