RAPGEF3 sirna

RAPGEF3 siRNA (Mouse)

Cat. Num. AAA828770

• Gene Names: Rapgef3; Epac; Epac1; 2310016P22Rik; 9330170P05Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RAPGEF3; RAPGEF3 siRNA (Mouse); EPAC; EPAC1; Rap guanine nucleotide exchange factor 3; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; EPAC 1; cAMP-regulated guanine nucleotide exchange factor I; cAMP-GEFI; RAPGEF3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: RAPGEF3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 926

• Applicable Applications for RAPGEF3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse RAPGEF3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RAPGEF3 sirna

siRNA to inhibit RAPGEF3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 295317405
• NCBI GeneID: 223864
• NCBI Accession #: NP_001171281.1
• NCBI GenBank Nucleotide #: NM_001177810.1
• UniProt Accession #: Q8VCC8; Q8BZK9; Q8R1R1
• Molecular Weight: 104,426 Da
• NCBI Official Full Name: rap guanine nucleotide exchange factor 3 isoform 1
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor (GEF) 3
• NCBI Official Symbol: Rapgef3
• NCBI Official Synonym Symbols: Epac; Epac1; 2310016P22Rik; 9330170P05Rik
• NCBI Protein Information: rap guanine nucleotide exchange factor 3
• UniProt Protein Name: Rap guanine nucleotide exchange factor 3
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: Rapgef3
• UniProt Synonym Gene Names: Epac; Epac1; EPAC 1; cAMP-GEFI
• UniProt Entry Name: RPGF3_MOUSE

Uniprot Description

Epac1: Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Widely expressed with highest levels in adult kidney, heart, thyroid and brain, and fetal kidney. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs; GEFs, misc.

Cellular Component: membrane; cytoplasm; intracellular; cAMP-dependent protein kinase complex

Molecular Function: Rap guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; nucleotide binding; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: positive regulation of syncytium formation by plasma membrane fusion; positive regulation of protein export from nucleus; positive regulation of angiogenesis; regulation of protein amino acid phosphorylation; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of stress fiber formation; angiogenesis; positive regulation of peptidyl-serine phosphorylation; Rap protein signal transduction; positive regulation of GTPase activity