PKP1 sirna

PKP1 siRNA (Human)

Cat. Num. AAA828457

• Gene Names: PKP1; B6P
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: PKP1; PKP1 siRNA (Human); Plakophilin-1; Band 6 protein; B6P; PKP1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: PKP1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 747

• Applicable Applications for PKP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human PKP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for PKP1 sirna

siRNA to inhibit PKP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 53729346
• NCBI GeneID: 5317
• NCBI Accession #: NP_000290.2
• NCBI GenBank Nucleotide #: NM_000299.3
• UniProt Accession #: Q13835; O00645; Q14CA0; Q15152
• Molecular Weight: 80,497 Da
• NCBI Official Full Name: plakophilin-1 isoform 1b
• NCBI Official Synonym Full Names: plakophilin 1
• NCBI Official Symbol: PKP1
• NCBI Official Synonym Symbols: B6P
• NCBI Protein Information: plakophilin-1
• UniProt Protein Name: Plakophilin-1
• Protein Family: Plakophilin
• UniProt Gene Name: PKP1
• UniProt Synonym Gene Names: B6P
• UniProt Entry Name: PKP1_HUMAN

NCBI Description

This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may be involved in molecular recruitment and stabilization during desmosome formation. Mutations in this gene have been associated with the ectodermal dysplasia/skin fragility syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

plakophilin 1: Seems to play a role in junctional plaques. Contributes to epidermal morphogenesis. Defects in PKP1 are the cause of ectodermal dysplasia- skin fragility syndrome (EDSFS); also known as McGrath syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDSFS is characterized by features of both cutaneous fragility and congenital ectodermal dysplasia affecting abnormalities in other epithelia or tissues. Desmosomes in the skin are small and poorly formed with widening of keratinocyte intercellular spaces and perturbed desmosome/keratin intermediate filament interactions. Belongs to the beta-catenin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: nucleoplasm; desmosome; intracellular membrane-bound organelle; plasma membrane; intermediate filament; nucleus

Molecular Function: protein binding; signal transducer activity; intermediate filament binding; structural constituent of epidermis; lamin binding

Biological Process: cell-cell adhesion; apoptosis; multicellular organismal development; intermediate filament bundle assembly; cell adhesion; signal transduction; cell structure disassembly during apoptosis

Disease: Ectodermal Dysplasia/skin Fragility Syndrome