KAT7 sirna

KAT7 siRNA (Mouse)

Cat. Num. AAA828411

• Gene Names: Kat7; Hbo1; Hboa; Myst2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: KAT7; KAT7 siRNA (Mouse); HBO1; MYST2; Histone acetyltransferase KAT7; Histone acetyltransferase binding to ORC1; Lysine acetyltransferase 7; MOZ. YBF2/SAS3. SAS2 and TIP60 protein 2; MYST-2; KAT7 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: KAT7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 581

• Applicable Applications for KAT7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse KAT7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for KAT7 sirna

siRNA to inhibit KAT7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 303521989
• NCBI GeneID: 217127
• NCBI Accession #: NP_001181933.1
• NCBI GenBank Nucleotide #: NM_001195004.1
• UniProt Accession #: Q5SVQ0; Q5SVQ1; Q5SVQ2; Q5SVQ3; Q5SVQ7; Q6PGC6; Q80Y65
• Molecular Weight: 60,251 Da
• NCBI Official Full Name: histone acetyltransferase KAT7 isoform 2
• NCBI Official Synonym Full Names: K(lysine) acetyltransferase 7
• NCBI Official Symbol: Kat7
• NCBI Official Synonym Symbols: Hbo1; Hboa; Myst2
• NCBI Protein Information: histone acetyltransferase KAT7
• UniProt Protein Name: Histone acetyltransferase KAT7
• Protein Family: Histone acetyltransferase
• UniProt Gene Name: Kat7
• UniProt Synonym Gene Names: Hbo1; Myst2; MYST-2
• UniProt Entry Name: KAT7_MOUSE

Uniprot Description

MYST2: a histone acetyltransferase that participates in DNA replication and the loading of the minichromosome maintenance (Mcm) complex onto chromatin. Is required for origin recognition complex (Orc) formation and replication licensing, and is responsible for much of the acetylation of histone H4. Interacts with MCM2 and ORC1L. Phosphorylated by Cdc2 during mitosis, creating a site that recruits Plk1, which in turn phosphorylates MYST2. Its phosphorylation by Plk1 during mitosis may be required for the formation of the prereplicative complex. Interacts with the androgen receptor (AR) in the presence of dihydrotestosterone. Specifically represses AR-mediated transcription.

Protein type: Acetyltransferase; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; EC 2.3.1.48

Cellular Component: nucleoplasm; cytoplasm; nucleus; histone acetyltransferase complex

Molecular Function: transferase activity; histone acetyltransferase activity; zinc ion binding; metal ion binding; transferase activity, transferring acyl groups; transferase activity, transferring groups other than amino-acyl groups; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin modification; DNA replication