CATSPER1 sirna

CATSPER1 siRNA (Mouse)

Cat. Num. AAA827911

• Gene Names: Catsper1; KSper; Catsper
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CATSPER1; CATSPER1 siRNA (Mouse); Cation channel sperm-associated protein 1; CatSper1; CATSPER1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CATSPER1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 686

• Applicable Applications for CATSPER1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CATSPER1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CATSPER1 sirna

siRNA to inhibit CATSPER1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 21314844
• NCBI GeneID: 225865
• NCBI Accession #: NP_647462.1
• NCBI GenBank Nucleotide #: NM_139301.2
• UniProt Accession #: Q91ZR5; Q4PZC3; Q4PZC4; Q4PZC5; Q4PZC6; Q4PZD2
• Molecular Weight: 78,706 Da
• NCBI Official Full Name: cation channel sperm-associated protein 1
• NCBI Official Synonym Full Names: cation channel, sperm associated 1
• NCBI Official Symbol: Catsper1
• NCBI Official Synonym Symbols: KSper; Catsper
• NCBI Protein Information: cation channel sperm-associated protein 1
• UniProt Protein Name: Cation channel sperm-associated protein 1
• Protein Family: Cation channel sperm-associated protein
• UniProt Gene Name: Catsper1
• UniProt Synonym Gene Names: CatSper1
• UniProt Entry Name: CTSR1_MOUSE

Uniprot Description

CATSPER1: Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte. Activated by extracellular progesterone and prostaglandins following the sequence: progesterone > PGF1-alpha = PGE1 > PGA1 > PGE2 >> PGD2. The primary effect of progesterone activation is to shift voltage dependence towards more physiological, negative membrane potentials; it is not mediated by metabotropic receptors and second messengers. Sperm capacitation enhances the effect of progesterone by providing additional negative shift. Also activated by the elevation of intracellular pH. Defects in CATSPER1 are the cause of spermatogenic failure type 7 (SPGF7). An infertility disorder characterized by non-motile sperm or sperm motility below the normal threshold, low sperm count, increased abnormally structured spermatozoa, and reduced semen volume. Belongs to the cation channel sperm-associated (TC 1.A.1.19) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: cell projection; membrane; integral to membrane; plasma membrane; cilium

Molecular Function: voltage-gated calcium channel activity; protein binding; calcium channel activity; ion channel activity; voltage-gated ion channel activity; calcium activated cation channel activity

Biological Process: sperm motility; fusion of sperm to egg plasma membrane; transport; multicellular organismal development; calcium ion transport; spermatogenesis; ion transport; regulation of calcium ion transport; cell differentiation; transmembrane transport