ATOH1 sirna

ATOH1 siRNA (Human)

Cat. Num. AAA827902

• Gene Names: ATOH1; ATH1; HATH1; MATH-1; bHLHa14
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATOH1; ATOH1 siRNA (Human); ATH1; BHLHA14; Protein atonal homolog 1; Class A basic helix-loop-helix protein 14; bHLHa14; Helix-loop-helix protein hATH-1; hATH1; ATOH1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ATOH1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 354

• Applicable Applications for ATOH1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ATOH1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATOH1 sirna

siRNA to inhibit ATOH1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4885075
• NCBI GeneID: 474
• NCBI Accession #: NP_005163.1
• NCBI GenBank Nucleotide #: NM_005172.1
• UniProt Accession #: Q92858; Q14CT9
• Molecular Weight: 38,160 Da
• NCBI Official Full Name: protein atonal homolog 1
• NCBI Official Synonym Full Names: atonal bHLH transcription factor 1
• NCBI Official Symbol: ATOH1
• NCBI Official Synonym Symbols: ATH1; HATH1; MATH-1; bHLHa14
• NCBI Protein Information: protein atonal homolog 1
• UniProt Protein Name: Protein atonal homolog 1
• Protein Family: Protein atonal
• UniProt Gene Name: ATOH1
• UniProt Synonym Gene Names: ATH1; BHLHA14; bHLHa14; hATH1
• UniProt Entry Name: ATOH1_HUMAN

NCBI Description

This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47. [provided by RefSeq, Jul 2008]

Uniprot Description

ATOH1: a conserved transcription factor that regulates secretory cell differentiation in colonic epithelium. Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. May play a role in the differentiation of subsets of neural cells by activating E box- dependent transcription . Efficient DNA binding requires dimerization with another bHLH protein. Loss of ATOH1 expression in the colon of mice initiates colon cancer. ATOH1 has been silenced in most human colon cancers, and reactivation of this gene in cultured human colon cancer cells is anti-proliferative and induces apoptosis.

Protein type: Cell development/differentiation; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 4q22

Cellular Component: nucleus

Molecular Function: protein dimerization activity; chromatin DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; axon guidance; inner ear morphogenesis; central nervous system development; auditory receptor cell fate determination; neuron migration; positive regulation of transcription from RNA polymerase II promoter; positive regulation of auditory receptor cell differentiation; cerebral cortex development; auditory receptor cell fate specification; positive regulation of neuron differentiation; negative regulation of apoptosis