INIP sirna

INIP siRNA (Human)

Cat. Num. AAA827707

• Gene Names: INIP; MISE; SOSSC; SSBIP1; C9orf80; HSPC043; hSSBIP1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: INIP; INIP siRNA (Human); C9orf80; SSBIP1; SOSS complex subunit C; INTS3- and NABP-interacting protein; Sensor of single-strand DNA complex subunit C; Sensor of ssDNA subunit C; SOSS-C; Single-stranded DNA-binding protein-interacting protein 1; SSB-interacting protein 1; hSSBIP1; INIP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: INIP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 104

• Applicable Applications for INIP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human INIP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for INIP sirna

siRNA to inhibit INIP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 24308269
• NCBI GeneID: 58493
• NCBI Accession #: NP_067041.1
• NCBI GenBank Nucleotide #: NM_021218.1
• UniProt Accession #: Q9NRY2; Q5VWJ7; Q96E04; Q9P090
• Molecular Weight: 4,904 Da
• NCBI Official Full Name: SOSS complex subunit C
• NCBI Official Synonym Full Names: INTS3 and NABP interacting protein
• NCBI Official Symbol: INIP
• NCBI Official Synonym Symbols: MISE; SOSSC; SSBIP1; C9orf80; HSPC043; hSSBIP1
• NCBI Protein Information: SOSS complex subunit C
• UniProt Protein Name: SOSS complex subunit C
• Protein Family: SOSS complex
• UniProt Gene Name: INIP
• UniProt Synonym Gene Names: C9orf80; SSBIP1; SOSS-C; SSB-interacting protein 1; hSSBIP1
• UniProt Entry Name: SOSSC_HUMAN

NCBI Description

SOSSC is a subunit of single-stranded DNA (ssDNA)-binding complexes involved in the maintenance of genome stability (Huang et al., 2009) [PubMed 19683501].[supplied by OMIM, Feb 2010]

Uniprot Description

INIP: Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. The SOSS complex associates with single- stranded DNA at DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination- dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways. Belongs to the SOSS-C family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: response to ionizing radiation; DNA repair; response to DNA damage stimulus