NCOR2 sirna

NCOR2 siRNA (Human)

Cat. Num. AAA827494

• Gene Names: NCOR2; SMRT; TRAC; CTG26; SMRTE; TRAC1; N-CoR2; TNRC14; TRAC-1; SMAP270; SMRTE-tau
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: NCOR2; NCOR2 siRNA (Human); CTG26; Nuclear receptor corepressor 2; N-CoR2; CTG repeat protein 26; SMAP270; Silencing mediator of retinoic acid and thyroid hormone receptor; SMRT; T3 receptor-associating factor; TRAC; Thyroid-. retinoic-acid-receptor-associated corepressor; NCOR2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: NCOR2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2458

• Applicable Applications for NCOR2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human NCOR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for NCOR2 sirna

siRNA to inhibit NCOR2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 331284176
• NCBI GeneID: 9612
• NCBI Accession #: NP_001070729.2
• NCBI GenBank Nucleotide #: NM_001077261.3
• UniProt Accession #: Q9Y618; O00613; O15416; O15421; Q13354; Q56D06; Q59GM0; Q9Y5U0
• Molecular Weight: 268,361 Da
• NCBI Official Full Name: nuclear receptor corepressor 2 isoform 2
• NCBI Official Synonym Full Names: nuclear receptor corepressor 2
• NCBI Official Symbol: NCOR2
• NCBI Official Synonym Symbols: SMRT; TRAC; CTG26; SMRTE; TRAC1; N-CoR2; TNRC14; TRAC-1; SMAP270; SMRTE-tau
• NCBI Protein Information: nuclear receptor corepressor 2
• UniProt Protein Name: Nuclear receptor corepressor 2
• Protein Family: Nuclear receptor corepressor
• UniProt Gene Name: NCOR2
• UniProt Synonym Gene Names: CTG26; N-CoR2; SMRT; TRAC
• UniProt Entry Name: NCOR2_HUMAN

NCBI Description

This gene encodes a nuclear receptor co-repressor that mediates transcriptional silencing of certain target genes. The encoded protein is a member of a family of thyroid hormone- and retinoic acid receptor-associated co-repressors. This protein acts as part of a multisubunit complex which includes histone deacetylases to modify chromatin structure that prevents basal transcriptional activity of target genes. Aberrant expression of this gene is associated with certain cancers. Alternate splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Apr 2011]

Uniprot Description

SMRT: Transcriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors. Interacts with BCL6, HDAC7 and C1D. Interacts with NR4A2; this interaction increases in the absence of PITX3. Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid (TR) and the retinoid acid receptors (RAR) in the absence of ligand, and may stabilize their interaction with TFIIB. Interacts directly with RARA in the absence of ligand; the interaction represses RARA activity. Interacts (isoform SRMT) with HDAC10. Interacts with MINT. Component of the N-Cor repressor complex, at least composed of NCOR1, NCOR2, HDAC3, TBL1X, TBL1R, CORO2A and GPS2. Interacts with CBFA2T3 and ATXN1L. Interacts with RARB; the interaction is weak and does not repress RARB transactivational activity. Regulated during cell cycle progression. Ubiquitous. High levels of expression are detected in lung, spleen and brain. Belongs to the N-CoR nuclear receptor corepressors family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: nucleoplasm; nuclear body; nuclear matrix; membrane; transcriptional repressor complex; nucleus

Molecular Function: protein binding; DNA binding; histone deacetylase binding; protein N-terminus binding; chromatin binding; Notch binding; transcription corepressor activity

Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; gene expression; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process