RECQL sirna

RECQL siRNA (Human)

Cat. Num. AAA827206

• Gene Names: RECQL; RecQ1; RECQL1
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: RECQL; RECQL siRNA (Human); RECQ1; RECQL1; ATP-dependent DNA helicase Q1; DNA helicase. RecQ-like type 1; RecQ1; DNA-dependent ATPase Q1; RecQ protein-like 1; RECQL sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: RECQL siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 649

• Applicable Applications for RECQL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human RECQL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for RECQL sirna

siRNA to inhibit RECQL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14591904
• NCBI GeneID: 5965
• NCBI Accession #: NP_002898.2
• NCBI GenBank Nucleotide #: NM_002907.3
• UniProt Accession #: P46063; A8K6G2
• Molecular Weight: 73,457 Da
• NCBI Official Full Name: ATP-dependent DNA helicase Q1
• NCBI Official Synonym Full Names: RecQ helicase-like
• NCBI Official Symbol: RECQL
• NCBI Official Synonym Symbols: RecQ1; RECQL1
• NCBI Protein Information: ATP-dependent DNA helicase Q1
• UniProt Protein Name: ATP-dependent DNA helicase Q1
• Protein Family: ATP-dependent DNA helicase
• UniProt Gene Name: RECQL
• UniProt Synonym Gene Names: RECQ1; RECQL1; RecQ1
• UniProt Entry Name: RECQ1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RecQ DNA helicase family. DNA helicases are enzymes involved in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Some members of this family are associated with genetic disorders with predisposition to malignancy and chromosomal instability. The biological function of this helicase has not yet been determined. Two alternatively spliced transcripts, which encode the same isoform but differ in their 5' and 3' UTRs, have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

RECQL: DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Interacts with EXO1 and MLH1. High expression in heart, lung, skeletal muscle and kidney, low expression in brain. Belongs to the helicase family. RecQ subfamily.

Protein type: EC 3.6.4.12; Helicase; DNA repair, damage

Chromosomal Location of Human Ortholog: 12p12

Cellular Component: nucleoplasm; membrane; nucleus

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA helicase activity; ATP-dependent 3'-5' DNA helicase activity; DNA binding; ATP binding

Biological Process: DNA strand renaturation; DNA replication; DNA repair; DNA duplex unwinding; DNA recombination