FLNB sirna

FLNB siRNA (Human)

Cat. Num. AAA827089

• Gene Names: FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FLNB; FLNB siRNA (Human); FLN1L; FLN3; TABP; TAP; Filamin-B; FLN-B; ABP-278; ABP-280 homolog; Actin-binding-like protein; Beta-filamin; Filamin homolog 1; Fh1; Filamin-3; Thyroid autoantigen; Truncated actin-binding protein; Truncated ABP; FLNB sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FLNB siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2633

• Applicable Applications for FLNB sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FLNB gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FLNB sirna

siRNA to inhibit FLNB expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 256222411
• NCBI GeneID: 2317
• NCBI Accession #: NP_001157789.1
• NCBI GenBank Nucleotide #: NM_001164317.1
• UniProt Accession #: O75369; Q13706; Q59EC2; Q60FE7; Q6MZJ1; Q8WXS9; Q8WXT0; B2ZZ83; B2ZZ84; B2ZZ85; C9JKE6; C9JMC4
• Molecular Weight: 276,939 Da
• NCBI Official Full Name: filamin-B isoform 1
• NCBI Official Synonym Full Names: filamin B, beta
• NCBI Official Symbol: FLNB
• NCBI Official Synonym Symbols: AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280
• NCBI Protein Information: filamin-B
• UniProt Protein Name: Filamin-B
• Protein Family: Filamin
• UniProt Gene Name: FLNB
• UniProt Synonym Gene Names: FLN1L; FLN3; TABP; TAP; FLN-B; Fh1; Truncated ABP
• UniProt Entry Name: FLNB_HUMAN

NCBI Description

This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

FLNB: a ubiquitous cytoskeletal protein that links actin filaments to membrane glycoproteins. Six alternatively spliced isoforms have been described. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoforms 1 and 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells, retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord and platelets. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer's disease. Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: focal adhesion; cytoplasm; stress fiber; plasma membrane; integral to membrane; cell cortex; Z disc; cytosol; actin cytoskeleton

Molecular Function: identical protein binding; protein binding; actin binding

Biological Process: skeletal muscle development; cytoskeletal anchoring; cytokine and chemokine mediated signaling pathway; actin cytoskeleton organization and biogenesis; signal transduction; cell differentiation

Disease: Larsen Syndrome; Boomerang Dysplasia; Atelosteogenesis, Type I; Spondylocarpotarsal Synostosis Syndrome; Atelosteogenesis, Type Iii