LAMA5 sirna

LAMA5 siRNA (Mouse)

Cat. Num. AAA827020

• Gene Names: Lama5; [a]5; AA408760; AA408762; AI853660; mKIAA0533; laminin-511
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LAMA5; LAMA5 siRNA (Mouse); Laminin subunit alpha-5; Laminin-10 subunit alpha; Laminin-11 subunit alpha; Laminin-15 subunit alpha; LAMA5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: LAMA5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 3718

• Applicable Applications for LAMA5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse LAMA5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LAMA5 sirna

siRNA to inhibit LAMA5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124487155
• NCBI GeneID: 16776
• NCBI Accession #: NP_001074640.1
• NCBI GenBank Nucleotide #: NM_001081171.2
• UniProt Accession #: Q61001; Q9JHQ6; A2ABW7
• Molecular Weight: 404,054 Da
• NCBI Official Full Name: laminin subunit alpha-5
• NCBI Official Synonym Full Names: laminin, alpha 5
• NCBI Official Symbol: Lama5
• NCBI Official Synonym Symbols: [a]5; AA408760; AA408762; AI853660; mKIAA0533; laminin-511
• NCBI Protein Information: laminin subunit alpha-5
• UniProt Protein Name: Laminin subunit alpha-5
• Protein Family: Laminin
• UniProt Gene Name: Lama5
• UniProt Entry Name: LAMA5_MOUSE

Uniprot Description

LAMA5: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Protein type: Motility/polarity/chemotaxis; Secreted; Extracellular matrix; Secreted, signal peptide

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; laminin-5 complex; laminin-1 complex; laminin-10 complex; extracellular region; basement membrane; basal lamina; nucleus

Molecular Function: integrin binding; protein binding; receptor binding

Biological Process: regulation of cell adhesion; integrin-mediated signaling pathway; muscle development; cell migration; regulation of embryonic development; regulation of cell migration; regulation of cell proliferation; odontogenesis of dentine-containing teeth; organ morphogenesis; morphogenesis of embryonic epithelium; hair follicle development; branching morphogenesis of a tube; ureteric bud branching; cilium biogenesis; cell adhesion; neural crest cell migration; morphogenesis of a polarized epithelium; lung development