WDR45 sirna

WDR45 siRNA (Mouse)

Cat. Num. AAA827018

• Gene Names: Wdr45; JM5; Sfc19; Wdrx1; C79260; WIPI-4; DXImx38e
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WDR45; WDR45 siRNA (Mouse); DXIMX38E; WDRX1; WIPI4; WD repeat domain phosphoinositide-interacting protein 4; WIPI-4; WD repeat domain X-linked 1; WD repeat-containing protein 45; WDR45 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: WDR45 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 360

• Applicable Applications for WDR45 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse WDR45 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WDR45 sirna

siRNA to inhibit WDR45 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 595763236
• NCBI GeneID: 54636
• NCBI Accession #: NP_001277721.1
• NCBI GenBank Nucleotide #: NM_001290792.1
• UniProt Accession #: Q91VM3; Q9CVF7; Q9JIG9; A2AEV3; A2AEV4
• Molecular Weight: 38,291 Da
• NCBI Official Full Name: WD repeat domain phosphoinositide-interacting protein 4 isoform a
• NCBI Official Synonym Full Names: WD repeat domain 45
• NCBI Official Symbol: Wdr45
• NCBI Official Synonym Symbols: JM5; Sfc19; Wdrx1; C79260; WIPI-4; DXImx38e
• NCBI Protein Information: WD repeat domain phosphoinositide-interacting protein 4
• UniProt Protein Name: WD repeat domain phosphoinositide-interacting protein 4
• Protein Family: WD repeat domain phosphoinositide-interacting protein
• UniProt Gene Name: Wdr45
• UniProt Synonym Gene Names: DXImx38e; Wdrx1; Wipi4; WIPI-4
• UniProt Entry Name: WIPI4_MOUSE

Uniprot Description

WDR45: a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This gene has a pseudogene at chromosome 4q31.3. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity and full-length nature of some variants have not been determined. [provided by RefSeq, Jul 2008]

Protein type: Autophagy

Cellular Component: extrinsic to membrane; pre-autophagosomal structure membrane; cytosol

Molecular Function: phosphatidylinositol 3-phosphate binding

Biological Process: mitochondrion degradation; protein amino acid lipidation; autophagy; autophagic vacuole formation; cellular response to nitrogen starvation