CINP sirna

CINP siRNA (Human)

Cat. Num. AAA826901

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CINP; CINP siRNA (Human); Cyclin-dependent kinase 2-interacting protein; CDK2-interacting protein; CINP sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: CINP siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 212

• Applicable Applications for CINP sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CINP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CINP sirna

siRNA to inhibit CINP expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 14249156
• NCBI GeneID: 51550
• NCBI Accession #: NP_116019.1
• NCBI GenBank Nucleotide #: NM_032630.2
• UniProt Accession #: Q9BW66; Q9NPF9; F5H7P3; F5H8A7
• Molecular Weight: 26,051 Da
• NCBI Official Full Name: cyclin-dependent kinase 2-interacting protein
• NCBI Official Synonym Full Names: cyclin-dependent kinase 2 interacting protein
• NCBI Official Symbol: CINP
• NCBI Protein Information: cyclin-dependent kinase 2-interacting protein
• UniProt Protein Name: Cyclin-dependent kinase 2-interacting protein
• Protein Family: Cyclin-dependent kinase 2-interacting protein
• UniProt Gene Name: CINP
• UniProt Synonym Gene Names: CDK2-interacting protein
• UniProt Entry Name: CINP_HUMAN

NCBI Description

The protein encoded by this gene is reported to be a component of the DNA replication complex as well as a genome-maintenance protein. It may interact with proteins important for replication initiation and has been shown to bind chromatin at the G1 phase of the cell cycle and dissociate from chromatin with replication initiation. It may also serve to regulate checkpoint signaling as part of the DNA damage response. [provided by RefSeq, Jul 2013]

Uniprot Description

CINP: Interacts with the components of the replication complex and 2 kinases, CDK2 and CDC7, thereby providing a functional and physical link between CDK2 and CDC7 during firing of the origins of replication. Regulates ATR-mediated checkpoint signaling. Belongs to the CINP family.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 14q32.31

Cellular Component: nucleus

Molecular Function: protein binding

Biological Process: cell division; DNA replication; cell cycle; DNA repair