OGG1 recombinant protein

Recombinant Human OGG1 Protein

Cat. Num. AAA8249531

• Gene Names: OGG1; HMMH; MUTM; OGH1; HOGG1
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Synonyms: OGG1; Recombinant Human OGG1 Protein; MMH; MUTM; OGH1; N-glycosylase/DNA lyase; OGG1 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 muM filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 1mM EDTA, 50% Glycerol, pH 7.8.
• Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMPAR ALLPRRMGHR TLASTPALWA SIPCPRSELR LDLVLPSGQS FRWREQSPAH WSGVLADQVW TLTQTEEQLH CTVYRGDKSQ ASRPTPDELE AVRKYFQLDV TLAQLYHHWG SVDSHFQEVA QKFQGVRLLR QDPIECLFSF ICSSNNNIAR ITGMVERLCQ AFGPRLIQLD DVTYHGFPSL QALAGPEVEA HLRKLGLGYR ARYVSASARA ILEEQGGLAW LQQLRESSYE EAHKALCILP GVGTKVADCI CLMALDKPQA VPVDVHMWHI AQRDYSWHPT TSQAKGPSPQ TNKELGNFFR SLWGPYAGWA QAVLFSADLR QSRHAQEPPA KRRKGSKGPE G
• Sequence Length: 345

• Applicable Applications for OGG1 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for OGG1 recombinant protein

Recombinant Human OGG1 Protein is produced by our E.coli expression system and the target gene encoding Met1-Gly345 is expressed with a GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4505495
• NCBI GeneID: 4968
• NCBI Accession #: NP_002533.1
• NCBI GenBank Nucleotide #: NM_002542.5
• UniProt Accession #: O15527; O00390; O00670; O00705; O14876; O95488; P78554; Q9BW42; Q9UIK0; Q9UIK1; Q9UIK2; A8K1E3
• Molecular Weight: 36,085 Da
• NCBI Official Full Name: N-glycosylase/DNA lyase isoform 1a
• NCBI Official Synonym Full Names: 8-oxoguanine DNA glycosylase
• NCBI Official Symbol: OGG1
• NCBI Official Synonym Symbols: HMMH; MUTM; OGH1; HOGG1
• NCBI Protein Information: N-glycosylase/DNA lyase
• UniProt Protein Name: N-glycosylase/DNA lyase
• Protein Family: N-glycosylase/DNA lyase
• UniProt Gene Name: OGG1
• UniProt Synonym Gene Names: MMH; MUTM; OGH1; AP lyase
• UniProt Entry Name: OGG1_HUMAN

NCBI Description

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]

Uniprot Description

OGG1: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3' to the lesion. Defects in OGG1 may be a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. Belongs to the type-1 OGG1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA repair, damage; Deoxyribonuclease; EC 4.2.99.18; Lyase

Chromosomal Location of Human Ortholog: 3p26.2

Cellular Component: nuclear matrix; nuclear speck; nucleoplasm; nucleus; protein complex

Molecular Function: damaged DNA binding; endonuclease activity; oxidized purine base lesion DNA N-glycosylase activity; oxidized purine DNA binding; protein binding

Biological Process: base-excision repair; depurination; depyrimidination; nucleotide-excision repair, DNA incision; regulation of protein import into nucleus, translocation; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to oxidative stress; response to radiation

Disease: Renal Cell Carcinoma, Nonpapillary