GZMB recombinant protein

Recombinant Mouse GZMB Protein

Cat. Num. AAA8249139

• Gene Names: Gzmb; GZB; CCP1; Ctla1; Ctla-1; AI553453; CCP-1/C11
• Reactivity: Mouse
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: GZMB; Recombinant Mouse GZMB Protein; CGL1; CSPB; CTLA1; GRB; Granzyme B; C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E; GZMB recombinant protein

• Host: Human cells
• Reactivity: Mouse
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 muM filtered solution of PBS, pH 7.4.
• Sequence: GEIIGGHEVK PHSRPYMALL SIKDQQPEAI CGGFLIREDF VLTAAHCEGS IINVTLGAHN IKEQEKTQQV IPMVKCIPHP DYNPKTFSND IMLLKLKSKA KRTRAVRPLN LPRRNVNVKP GDVCYVAGWG RMAPMGKYSN TLQEVELTVQ KDRECESYFK NRYNKTNQIC AGDPKTKRAS FRGDSGGPLV CKKVAAGIVS YGYKDGSPPR AFTKVSSFLS WIKKTMKSSV DHHHHHH
• Sequence Length: 247

• Applicable Applications for GZMB recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for GZMB recombinant protein

Recombinant Mouse GZMB Protein is produced by our mammalian expression system and the target gene encoding Gly19-Ser247 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 7305123
• NCBI GeneID: 14939
• NCBI Accession #: NP_038570.1
• NCBI GenBank Nucleotide #: NM_013542.3
• UniProt Accession #: P04187
• Molecular Weight: 27,470 Da
• NCBI Official Full Name: granzyme B(G,H) preproprotein
• NCBI Official Synonym Full Names: granzyme B
• NCBI Official Symbol: Gzmb
• NCBI Official Synonym Symbols: GZB; CCP1; Ctla1; Ctla-1; AI553453; CCP-1/C11
• NCBI Protein Information: granzyme B(G,H)
• UniProt Protein Name: Granzyme B(G,H)
• Protein Family: Granzyme
• UniProt Gene Name: Gzmb
• UniProt Synonym Gene Names: Ctla-1; Ctla1; CCP1
• UniProt Entry Name: GRAB_MOUSE

NCBI Description

This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Mice lacking a functional copy of this gene exhibit impaired immune cell-mediated cytolysis. [provided by RefSeq, Sep 2016]

Uniprot Description

GZMB: This enzyme is necessary for target cell lysis in cell- mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. By staphylococcal enterotoxin A (SEA) in peripheral blood leukocytes. Inactivated by the serine protease inhibitor diisopropylfluorophosphate. Belongs to the peptidase S1 family. Granzyme subfamily.

Protein type: Apoptosis; EC 3.4.21.79; Protease

Cellular Component: cytoplasm

Molecular Function: protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: induction of apoptosis by granzyme; protein processing; T cell mediated cytotoxicity