GADD45B recombinant protein

Recombinant Human GADD45B Protein

Cat. Num. AAA8249081

• Gene Names: GADD45B; MYD118; GADD45BETA
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: GADD45B; Recombinant Human GADD45B Protein; MYD118; Growth arrest and DNA damage-inducible protein GADD45 beta; Myeloid differentiation primary response protein MyD118; Negative growth regulatory protein MyD118; GADD45B recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence: MGSSHHHHHH SSGLVPRGSH MTLEELVACD NAAQKMQTVT AAVEELLVAA QRQDRLTVGV YESAKLMNVD PDSVVLCLLA IDEEEEDDIA LQIHFTLIQS FCCDNDINIV RVSGMQRLAQ LLGEPAETQG TTEARDLHCL LVTNPHTDAW KSHGLVEVAS YCEESRGNNQ WVPYISLQER
• Sequence Length: 160

• Applicable Applications for GADD45B recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for GADD45B recombinant protein

Recombinant Human GADD45B Protein is produced by our E Coli expression system and the target gene encoding Met1-Arg160 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 86991436
• NCBI GeneID: 4616
• NCBI Accession #: NP_056490.2
• NCBI GenBank Nucleotide #: NM_015675.3
• UniProt Accession #: O75293; O75960; Q17R46; A8KAM2
• Molecular Weight: 17,818 Da
• NCBI Official Full Name: growth arrest and DNA damage-inducible protein GADD45 beta
• NCBI Official Synonym Full Names: growth arrest and DNA damage inducible beta
• NCBI Official Symbol: GADD45B
• NCBI Official Synonym Symbols: MYD118; GADD45BETA
• NCBI Protein Information: growth arrest and DNA damage-inducible protein GADD45 beta
• UniProt Protein Name: Growth arrest and DNA damage-inducible protein GADD45 beta
• Protein Family: Growth arrest and DNA damage-inducible protein
• UniProt Gene Name: GADD45B
• UniProt Synonym Gene Names: MYD118
• UniProt Entry Name: GA45B_HUMAN

NCBI Description

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The genes in this group respond to environmental stresses by mediating activation of the p38/JNK pathway. This activation is mediated via their proteins binding and activating MTK1/MEKK4 kinase, which is an upstream activator of both p38 and JNK MAPKs. The function of these genes or their protein products is involved in the regulation of growth and apoptosis. These genes are regulated by different mechanisms, but they are often coordinately expressed and can function cooperatively in inhibiting cell growth. [provided by RefSeq, Jul 2008]

Uniprot Description

GADD45B: Involved in the regulation of growth and apoptosis. Mediates activation of stress-responsive MTK1/MEKK4 MAPKKK. Belongs to the GADD45 family.

Protein type: Apoptosis; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: activation of MAPKKK activity; positive regulation of apoptosis; positive regulation of JNK cascade; regulation of cell cycle