EFNB1 recombinant protein

Recombinant Mouse EFNB1 Protein

Cat. Num. AAA8248960

• Gene Names: Efnb1; Epl2; EFL-3; Elk-L; Eplg2; Lerk2; Stra1; Cek5-L; LERK-2
• Reactivity: Mouse
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: EFNB1; Recombinant Mouse EFNB1 Protein; EFL3; EPLG2; LERK2; Ephrin-B1; EFL-3; ELK ligand; ELK-L; EPH-related receptor tyrosine kinase ligand 2; LERK-2; EFNB1 recombinant protein

• Host: Human cells
• Reactivity: Mouse
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence: KNLEPVSWSS LNPKFLSGKG LVIYPKIGDK LDIICPRAEA GRPYEYYKLY LVRPEQAAAC STVLDPNVLV TCNKPHQEIR FTIKFQEFSP NYMGLEFKKY HDYYITSTSN GSLEGLENRE GGVCRTRTMK IVMKVGQDPN AVTPEQLTTS RPSKESDNTV KTATQAPGRG SQGDSDGKHE TVNQEEKSGP GAGGGGSGDS VDDIEGRMDE PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRE EMTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG KHHHHHH
• Sequence Length: 345

• Applicable Applications for EFNB1 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for EFNB1 recombinant protein

Recombinant Mouse EFNB1 Protein is produced by our mammalian expression system and the target gene encoding Lys30-Ser229 is expressed with a Fc, 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6753726
• NCBI GeneID: 13641
• NCBI Accession #: NP_034240.1
• NCBI GenBank Nucleotide #: NM_010110.5
• UniProt Accession #: P52795
• Molecular Weight: 37,859 Da
• NCBI Official Full Name: ephrin-B1
• NCBI Official Synonym Full Names: ephrin B1
• NCBI Official Symbol: Efnb1
• NCBI Official Synonym Symbols: Epl2; EFL-3; Elk-L; Eplg2; Lerk2; Stra1; Cek5-L; LERK-2
• NCBI Protein Information: ephrin-B1
• UniProt Protein Name: Ephrin-B1
• Protein Family: Ephrin
• UniProt Gene Name: Efnb1
• UniProt Synonym Gene Names: Epl2; Eplg2; Lerk2; Stra1; CEK5-L; ELK-L; LERK-2
• UniProt Entry Name: EFNB1_MOUSE

NCBI Description

This gene encodes a membrane protein that acts as a ligand for Eph family receptors. Signalling occurs bidirectionally in both the cell containing the receptor and the cell expressing this protein. Activity of this protein is important in neuronal axon growth and other developmental processes. [provided by RefSeq, May 2015]

Uniprot Description

EFNB1: a type I membrane protein of the ephrin family. A ligand of Eph-related receptor tyrosine kinases EphB1 and EphA1. Ephrins and ephrin receptors mediate numerous developmental processes, particularly in the nervous system. Ephrin-B1 may play a role in cell adhesion and functions in the development or maintenance of the nervous system. Binding to its receptor induces the collapse of commissural axons/growth cones in vitro. Induced by TNF-alpha. Expressed in brain, heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas.

Protein type: Ligand, receptor tyrosine kinase; Membrane protein, integral

Cellular Component: cytoplasm; lipid raft; nucleus; plasma membrane; synapse

Molecular Function: ephrin receptor binding; protein binding

Biological Process: axon guidance; embryonic pattern specification; ephrin receptor signaling pathway; nervous system development; neural crest cell migration; positive regulation of T cell proliferation; T cell costimulation