COL8A1 recombinant protein
Recombinant Human COL8A1 Protein
Gene Names
COL8A1; C3orf7
Reactivity
Human
Applications
ELISA, Western Blot, SDS-Page
Purity
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Synonyms
COL8A1; Recombinant Human COL8A1 Protein; C3orf7; Collagen alpha-1(VIII) chain; Endothelial collagen; COL8A1 recombinant protein
Host
Human cells
Reactivity
Human
Purity/Purification
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Form/Format
Lyophilized from a 0.2 muM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
Sequence
GAYYGIKPLP PQIPPQMPPQ IPQYQPLGQQ VPHMPLAKDG LAMGKEMPHL QYGKEYPHLP QYMKEIQPAP RMGKEAVPKK GKEIPLASLR GEQGPRGEPG PRGPPGPPGL PGHGIPGIKG KPGPQGYPGV GKPGMPGMPG KPGAMGMPGA KGEIGQKGEI GPMGIPGPQG PPGPHGLPGI GKPGGPGLPG QPGPKGDRGP KGLPGPQGLR GPKGDKGFGM PGAPGVKGPP GMHGPPGPVG LPGVGKPGVT GFPGPQGPLG KPGAPGEPGP QGPIGVPGVQ GPPGIPGIGK PGQDGIPGQP GFPGGKGEQG LPGLPGPPGL PGIGKPGFPG PKGDRGMGGV PGALGPRGEK GPIGAPGIGG PPGEPGLPGI PGPMGPPGAI GFPGPKGEGG IVGPQGPPGP KGEPGLQGFP GKPGFLGEVG PPGMRGLPGP IGPKGEAGQK GVPGLPGVPG LLGPKGEPGI PGDQGLQGPP GIPGIGGPSG PIGPPGIPGP KGEPGLPGPP GFPGIGKPGV AGLHGPPGKP GALGPQGQPG LPGPPGPPGP PGPPAVMPPT PPPQGEYLPD MGLGIDGVKP PHAYGAKKGK NGGPAYEMPA FTAELTAPFP PVGAPVKFNK LLYNGRQNYN PQTGIFTCEV PGVYYFAYHV HCKGGNVWVA LFKNNEPVMY TYDEYKKGFL DQASGSAVLL LRPGDRVFLQ MPSEQAAGLY AGQYVHSSFS GYLLYPMVDH HHHHH
Sequence Length
744
Applicable Applications for COL8A1 recombinant protein
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
Endotoxin
Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
Directions for Use
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Preparation and Storage
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
Related Product Information for COL8A1 recombinant protein
Recombinant Human COL8A1 Protein is produced by our Mammalian expression system and the target gene encoding Gly28-Met744 is expressed with a 6His tag at the C-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73,364 Da
NCBI Official Full Name
collagen alpha-1(VIII) chain
NCBI Official Synonym Full Names
collagen type VIII alpha 1 chain
NCBI Official Symbol
COL8A1
NCBI Official Synonym Symbols
C3orf7
NCBI Protein Information
collagen alpha-1(VIII) chain
UniProt Protein Name
Collagen alpha-1(VIII) chain
Protein Family
UniProt Gene Name
COL8A1
UniProt Synonym Gene Names
C3orf7
UniProt Entry Name
CO8A1_HUMAN
NCBI Description
This gene encodes one of the two alpha chains of type VIII collagen. The gene product is a short chain collagen and a major component of the basement membrane of the corneal endothelium. The type VIII collagen fibril can be either a homo- or a heterotrimer. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Dec 2011]
Uniprot Description
COL8A1: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis.
Protein type: Extracellular matrix; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 3q12.3
Cellular Component: collagen type VIII; endoplasmic reticulum lumen; extracellular matrix; extracellular region; intracellular membrane-bound organelle
Molecular Function: protein binding
Biological Process: collagen catabolic process; extracellular matrix organization and biogenesis
Research Articles on COL8A1
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Product Notes
The COL8A1 col8a1 (Catalog #AAA8248813) is a Recombinant Protein produced from Human cells and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Human COL8A1 Protein reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COL8A1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS). Researchers should empirically determine the suitability of the COL8A1 col8a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GAYYGIKPLP PQIPPQMPPQ IPQYQPLGQQ VPHMPLAKDG LAMGKEMPHL QYGKEYPHLP QYMKEIQPAP RMGKEAVPKK GKEIPLASLR GEQGPRGEPG PRGPPGPPGL PGHGIPGIKG KPGPQGYPGV GKPGMPGMPG KPGAMGMPGA KGEIGQKGEI GPMGIPGPQG PPGPHGLPGI GKPGGPGLPG QPGPKGDRGP KGLPGPQGLR GPKGDKGFGM PGAPGVKGPP GMHGPPGPVG LPGVGKPGVT GFPGPQGPLG KPGAPGEPGP QGPIGVPGVQ GPPGIPGIGK PGQDGIPGQP GFPGGKGEQG LPGLPGPPGL PGIGKPGFPG PKGDRGMGGV PGALGPRGEK GPIGAPGIGG PPGEPGLPGI PGPMGPPGAI GFPGPKGEGG IVGPQGPPGP KGEPGLQGFP GKPGFLGEVG PPGMRGLPGP IGPKGEAGQK GVPGLPGVPG LLGPKGEPGI PGDQGLQGPP GIPGIGGPSG PIGPPGIPGP KGEPGLPGPP GFPGIGKPGV AGLHGPPGKP GALGPQGQPG LPGPPGPPGP PGPPAVMPPT PPPQGEYLPD MGLGIDGVKP PHAYGAKKGK NGGPAYEMPA FTAELTAPFP PVGAPVKFNK LLYNGRQNYN PQTGIFTCEV PGVYYFAYHV HCKGGNVWVA LFKNNEPVMY TYDEYKKGFL DQASGSAVLL LRPGDRVFLQ MPSEQAAGLY AGQYVHSSFS GYLLYPMVDH HHHHH. It is sometimes possible for the material contained within the vial of "COL8A1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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