CASK recombinant protein

Recombinant Human CASK Protein

Cat. Num. AAA8248664

• Gene Names: CASK; CMG; FGS4; LIN2; TNRC8; CAGH39; CAMGUK; MICPCH; MRXSNA
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: CASK; Recombinant Human CASK Protein; LIN2; Peripheral plasma membrane protein CASK; hCASK; Calcium/calmodulin-dependent serine protein kinase; Protein lin-2 homolog; CASK recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence: MADDDVLFED VYELCEVIGK GPFSVVRRCI NRETGQQFAV KIVDVAKFTS SPGLSTEDLK REASICHMLK HPHIVELLET YSSDGMLYMV FEFMDGADLC FEIVKRADAG FVYSEAVASH YMRQILEALR YCHDNNIIHR DVKPHCVLLA SKENSAPVKL GGFGVAIQLG ESGLVAGGRV GTPHFMAPEV VKREPYGKPV DVWGCGVILF ILLSGCLPFY GTKERLFEGI IKGKYKMNPR QWSHISESAK DLVRRMLMLD PAERITVYEA LNHPWLKERD RYAYKIHLPE TVEQLRKFNA RRKLKGAVLA AVSSHKFNSF YGDPPEELPD FSEDPTSSGL LAAERAVSQV LDSLEEIHAL TDCSEKDLDF LHSVFQDQHL HTLLDLYDKI NTKSSPQIRN PPSDAVQRAK EVLEEISCYP ENNDAKELKR ILTQPHFMAL LQTHDVVAHE VYSDEALRVT PPPTSPYLNG DSPESANGDM DMENVTRVRL VQFQKNTDEP MGITLKMNEL NHCIVARIMH GGMIHRQGTL HVGDEIREIN GISVANQTVE QLQKMLREMR GSITFKIVPS YRTQSSSCED LPSTTQPKGR QIYVRAQFEY DPAKDDLIPC KEAGIRFRVG DIIQIISKDD HNWWQGKLEN SKNGTAGLIP SPELQEWRVA CIAMEKTKQE QQASCTWFGK KKKQYKDKYL AKHNADLVTY EEVVKLPAFK RKTLVLLGAH GVGRRHIKNT LITKHPDRFA YPIPHTTRPP KKDEENGKNY YFVSHDQMMQ DISNNEYLEY GSHEDAMYGT KLETIRKIHE QGLIAILDVE PQALKVLRTA EFAPFVVFIA APTITPGLNE DESLQRLQKE SDILQRTYAH YFDLTIINNE IDETIRHLEE AVELVCTAPQ WVPVSWVYVD HHHHHH
• Sequence Length: 898

• Applicable Applications for CASK recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CASK recombinant protein

Recombinant Human CASK Protein is produced by our mammalian expression system and the target gene encoding Met1-Tyr898 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 186700627
• NCBI GeneID: 8573
• NCBI Accession #: NP_001119526.1
• NCBI GenBank Nucleotide #: NM_001126054.2
• UniProt Accession #: O14936; O43215; Q17RI4; Q59HA0; Q5VT16; Q5VT17; Q5VT18; Q5VT19; Q66T42; Q9BYH6; A6NES1; B7ZKY0
• Molecular Weight: 103,211 Da
• NCBI Official Full Name: peripheral plasma membrane protein CASK isoform 2
• NCBI Official Synonym Full Names: calcium/calmodulin dependent serine protein kinase
• NCBI Official Symbol: CASK
• NCBI Official Synonym Symbols: CMG; FGS4; LIN2; TNRC8; CAGH39; CAMGUK; MICPCH; MRXSNA
• NCBI Protein Information: peripheral plasma membrane protein CASK
• UniProt Protein Name: Peripheral plasma membrane protein CASK
• Protein Family: Caskin
• UniProt Gene Name: CASK
• UniProt Synonym Gene Names: LIN2; hCASK
• UniProt Entry Name: CSKP_HUMAN

NCBI Description

This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, mental retardation and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked mental retardation. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]

Uniprot Description

CASK: a MAGUK family protein and a protein kinase of the CAMK group. Contains one protein kinase domain, one guanylate kinase domain, two L27 domains, one PDZ domain and one SH3 domain. Plays a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. Lacks the canonical DFG motif required for Mg++ binding, but retains protein kinase activity even without binding Mg++. Six alternatively spliced human isoforms have been described.

Protein type: Adaptor/scaffold; CAMK group; CASK family; EC 2.7.11.1; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: actin cytoskeleton; basement membrane; cytoplasm; cytosol; focal adhesion; intercellular junction; nuclear lamina; nuclear matrix; nucleolus; plasma membrane; presynaptic membrane

Molecular Function: guanylate kinase activity; protein binding

Biological Process: cell adhesion; negative regulation of cell-matrix adhesion; neurotransmitter secretion

Disease: Fg Syndrome 4; Mental Retardation And Microcephaly With Pontine And Cerebellar Hypoplasia