ADPRH recombinant protein
Recombinant Human ADPRH Protein
Gene Names
ADPRH; ARH1
Reactivity
Human
Applications
ELISA, Western Blot, SDS-Page
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Synonyms
ADPRH; Recombinant Human ADPRH Protein; ARH1; [Protein ADP-ribosylarginine] hydrolase; ADP-ribosylarginine hydrolase; ADP-ribose-L-arginine cleaving enzyme; ADPRH recombinant protein
Host
E Coli
Reactivity
Human
Purity/Purification
Greater than 95% as determined by reducing SDS-PAGE.
Form/Format
Supplied as a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, 50% Glycerol, pH 7.4.
Sequence
MGSSHHHHHH SSGLVPRGSH MEKYVAAMVL SAAGDALGYY NGKWEFLQDG EKIHRQLAQL GGLDALDVGR WRVSDDTVMH LATAEALVEA GKAPKLTQLY YLLAKHYQDC MEDMDGRAPG GASVHNAMQL KPGKPNGWRI PFNSHEGGCG AAMRAMCIGL RFPHHSQLDT LIQVSIESGR MTHHHPTGYL GALASALFTA YAVNSRPPLQ WGKGLMELLP EAKKYIVQSG YFVEENLQHW SYFQTKWENY LKLRGILDGE SAPTFPESFG VKERDQFYTS LSYSGWGGSS GHDAPMIAYD AVLAAGDSWK ELAHRAFFHG GDSDSTAAIA GCWWGVMYGF KGVSPSNYEK LEYRNRLEET ARALYSLGSK EDTVISL
Sequence Length
357
Applicable Applications for ADPRH recombinant protein
ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
Endotoxin
Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
Directions for Use
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Preparation and Storage
Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.
Related Product Information for ADPRH recombinant protein
Recombinant Human ADPRH Protein is produced by our E Coli expression system and the target gene encoding Met1-Leu357 is expressed with a 6His tag at the N-terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,507 Da
NCBI Official Full Name
ADP-ribosylarginine hydrolase isoform 1
NCBI Official Synonym Full Names
ADP-ribosylarginine hydrolase
NCBI Official Symbol
ADPRH
NCBI Official Synonym Symbols
ARH1
NCBI Protein Information
ADP-ribosylarginine hydrolase
UniProt Protein Name
[Protein ADP-ribosylarginine] hydrolase
Protein Family
UniProt Gene Name
ADPRH
UniProt Synonym Gene Names
ARH1; ADP-ribosylarginine hydrolase
UniProt Entry Name
ADPRH_HUMAN
NCBI Description
The enzyme encoded by this gene catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. Unlike the rat and mouse enzymes that require DTT for maximal activity, the human enzyme is DTT-independent. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, May 2014]
Uniprot Description
ADPRH: Catalyzes the reverse reaction of mono-ADP-ribosylation. Belongs to the ADP-ribosylglycohydrolase family.
Protein type: EC 3.2.2.19; Hydrolase
Chromosomal Location of Human Ortholog: 3q13.31-q13.33
Cellular Component: endomembrane system; intracellular
Molecular Function: ADP-ribosylarginine hydrolase activity; GTPase activator activity; protein binding; Rab GTPase binding
Biological Process: intracellular protein transport; protein modification process; regulation of vesicle fusion
Research Articles on ADPRH
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Product Notes
The ADPRH adprh (Catalog #AAA8248515) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Human ADPRH Protein reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ADPRH can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS). Researchers should empirically determine the suitability of the ADPRH adprh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MEKYVAAMVL SAAGDALGYY NGKWEFLQDG EKIHRQLAQL GGLDALDVGR WRVSDDTVMH LATAEALVEA GKAPKLTQLY YLLAKHYQDC MEDMDGRAPG GASVHNAMQL KPGKPNGWRI PFNSHEGGCG AAMRAMCIGL RFPHHSQLDT LIQVSIESGR MTHHHPTGYL GALASALFTA YAVNSRPPLQ WGKGLMELLP EAKKYIVQSG YFVEENLQHW SYFQTKWENY LKLRGILDGE SAPTFPESFG VKERDQFYTS LSYSGWGGSS GHDAPMIAYD AVLAAGDSWK ELAHRAFFHG GDSDSTAAIA GCWWGVMYGF KGVSPSNYEK LEYRNRLEET ARALYSLGSK EDTVISL. It is sometimes possible for the material contained within the vial of "ADPRH, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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