Typical Testing Data/Standard Curve (for reference only)
Mouse CCL21 ELISA Kit | CCL21A elisa kit
Mouse CCL21 ELISA Kit
Gene Names
Gm1987; 100038965
Reactivity
Mouse
Applications
ELISA
Synonyms
CCL21; Mouse CCL21 ELISA Kit; Scya21; Scya21a; C-C motif chemokine 21a; 6Ckine; Beta-chemokine exodus-2; Small-inducible cytokine A21a; Thymus-derived chemotactic agent 4; TCA4; CCL21A elisa kit
Reactivity
Mouse
Specificity
The Mouse CCL21 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CCL21 proteins within the range of 31.2 pg/ml - 2000 pg/ml.
Sequence Length
133
Applicable Applications for CCL21A elisa kit
Sandwich ELISA (SE)
Samples
Cell culture supernatants, serum, plasma
Sensitivity
< 15 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CCL21A elisa kit
Principle of the Assay: The MyBioSource' Mouse CCL21 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CCL21 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse CCL21 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CCL21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.
Background/Introduction: Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,558 Da
NCBI Official Full Name
predicted gene 1987
NCBI Official Synonym Full Names
predicted gene 1987
NCBI Official Symbol
Gm1987
NCBI Official Synonym Symbols
100038965
NCBI Protein Information
predicted gene 1987
UniProt Protein Name
C-C motif chemokine 21a
Protein Family
UniProt Gene Name
Ccl21a
UniProt Synonym Gene Names
Scya21; Scya21a; TCA4
UniProt Entry Name
CC21A_MOUSE
Uniprot Description
CCL21a: Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Potent mesangial cell chemoattractant. Shows preferential activity towards naive T- cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Mice carrying an autosomal recessive mutation designated paucity of lymph node T-cells (plt) show dramatically reduced numbers of T-cells in lymph nodes, Peyer patches, and the white pulp of the spleen. Plt seems to correspond to Scya21b. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Chemokine
Molecular Function: chemokine activity; chemokine receptor binding
Biological Process: lymph node development; induction of positive chemotaxis
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Product Notes
The Mouse CCL21A ccl21a (Catalog #AAA824848) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA824848 ELISA Kit recognizes Mouse CCL21A. AAA Biotech's CCL21 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (SE). Researchers should empirically determine the suitability of the CCL21A ccl21a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCL21, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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