Mouse FcRn ELISA Kit | FCGRT elisa kit

Mouse FcRn ELISA Kit

Cat. Num. AAA8248436

• Gene Names: Fcgrt; FcRn
• Reactivity: Mouse
• Synonyms: FcRn; Mouse FcRn ELISA Kit; FCRN; IgG receptor FcRn large subunit p51; IgG Fc fragment receptor transporter alpha chain; Neonatal Fc receptor; FCGRT elisa kit

• Reactivity: Mouse
• Specificity: The Mouse FcRn ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse FcRn proteins within the range of 93.7 pg/ml - 6000 pg/ml.
• Sequence Length: 365

• Samples: Cell Culture Supernates, Serum, Plasma, Cell Lysates
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FCGRT elisa kit

Principle of the Assay: The Mouse FcRn ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse FcRn in Cell Culture Supernates, Serum, Plasma, Cell Lysates. This assay employs an antibody specific for Mouse FcRn coated on a 96-well plate. Standards and samples are pipetted into the wells and FcRn present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse FcRn antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FcRn bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: The neonatal Fc receptor (FcRn), also known as the Brambell receptor, is a protein that in humans is encoded by the FCGRT gene. The neonatal Fc receptor is an Fc receptor which is similar in structure to the MHC class I molecule and also associates with beta-2-microglobulin. It was first discovered in rodents as a unique receptor capable of transporting IgG from mother's milk across the epithelium of newborn rodent's gut into the newborn's bloodstream. Further studies revealed a similar receptor in humans, leading to the naming as a neonatal Fc receptor. In humans, however, it is found in the placenta to help facilitate transport of mother's IgG to the growing fetus and it has also been shown to play a role in monitoring IgG turnover. Neonatal Fc receptor expression is up-regulated by the proinflammatory cytokine,TNF-alpha, and down-regulated by IFN-gamma.

NCBI and Uniprot Product Information

• NCBI GI #: 170172528
• NCBI GeneID: 14132
• NCBI Accession #: NP_034319.2
• NCBI GenBank Nucleotide #: NM_010189.3
• UniProt Accession #: Q61559; Q9QUR0; Q9R2A5
• Molecular Weight: 40,093 Da
• NCBI Official Full Name: IgG receptor FcRn large subunit p51
• NCBI Official Synonym Full Names: Fc receptor, IgG, alpha chain transporter
• NCBI Official Symbol: Fcgrt
• NCBI Official Synonym Symbols: FcRn
• NCBI Protein Information: IgG receptor FcRn large subunit p51
• UniProt Protein Name: IgG receptor FcRn large subunit p51
• Protein Family: IgG receptor
• UniProt Gene Name: Fcgrt
• UniProt Synonym Gene Names: Fcrn; FcRn
• UniProt Entry Name: FCGRN_MOUSE

Uniprot Description

FCGRT: Binds to the Fc region of monomeric immunoglobulins gamma. Mediates the uptake of IgG from milk. Possible role in transfer of immunoglobulin G from mother to fetus. Belongs to the immunoglobulin superfamily.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Receptor, misc.

Cellular Component: plasma membrane

Molecular Function: antigen binding; beta-2-microglobulin binding; IgG binding; IgG receptor activity

Biological Process: antigen processing and presentation