Typical Testing Data/Standard Curve (for reference only)
(Mouse FCRN/FCGRT PicoKine ELISA Kit standard curve )
Mouse FCRN/FCGRT ELISA Kit | FCGRT elisa kit
Mouse FCRN/FCGRT PicoKine ELISA Kit
Gene Names
Fcgrt; FcRn
Reactivity
Mouse
Synonyms
FCRN/FCGRT; Mouse FCRN/FCGRT PicoKine ELISA Kit; FCGRT; FcRn alpha chain; FcRn; IgG Gc receptor; Neonatal Fc receptor; Q61559; IgG receptor FcRn large subunit p51; Fc fragment of IgG receptor and transporter; FCGRT elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse FCRN/FCGRT
Sequence Length
365
Samples
Cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates.
Assay Type
Sandwich
Detection Range
93.7pg/ml-6000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Typical Testing Data/Standard Curve (for reference only)
(Mouse FCRN/FCGRT PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse FCRN/FCGRT PicoKine ELISA Kit standard curve )
Related Product Information for FCGRT elisa kit
Principle of the Assay: Mouse FCRN/FCGRT ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for FCRN/FCGRT has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: S22-S297) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FCRN/FCGRT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse FCRN/FCGRT amount of sample captured in plate.
Background/Introduction: The neonatal Fc receptor (FcRn), also known as the Brambell receptor, is a protein that in humans is encoded by the FCGRT gene. The neonatal Fc receptor is an Fc receptor which is similar in structure to the MHC class I molecule and also associates with beta-2-microglobulin. It was first discovered in rodents as a unique receptor capable of transporting IgG from mother's milk across the epithelium of newborn rodent's gut into the newborn's bloodstream. Further studies revealed a similar receptor in humans, leading to the naming as a neonatal Fc receptor. In humans, however, it is found in the placenta to help facilitate transport of mother's IgG to the growing fetus and it has also been shown to play a role in monitoring IgG turnover. Neonatal Fc receptor expression is up-regulated by the proinflammatory cytokine,TNF-alpha, and down-regulated by IFN-gamma.
Background/Introduction: The neonatal Fc receptor (FcRn), also known as the Brambell receptor, is a protein that in humans is encoded by the FCGRT gene. The neonatal Fc receptor is an Fc receptor which is similar in structure to the MHC class I molecule and also associates with beta-2-microglobulin. It was first discovered in rodents as a unique receptor capable of transporting IgG from mother's milk across the epithelium of newborn rodent's gut into the newborn's bloodstream. Further studies revealed a similar receptor in humans, leading to the naming as a neonatal Fc receptor. In humans, however, it is found in the placenta to help facilitate transport of mother's IgG to the growing fetus and it has also been shown to play a role in monitoring IgG turnover. Neonatal Fc receptor expression is up-regulated by the proinflammatory cytokine,TNF-alpha, and down-regulated by IFN-gamma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,093 Da
NCBI Official Full Name
IgG receptor FcRn large subunit p51
NCBI Official Synonym Full Names
Fc receptor, IgG, alpha chain transporter
NCBI Official Symbol
Fcgrt
NCBI Official Synonym Symbols
FcRn
NCBI Protein Information
IgG receptor FcRn large subunit p51
UniProt Protein Name
IgG receptor FcRn large subunit p51
Protein Family
UniProt Gene Name
Fcgrt
UniProt Synonym Gene Names
Fcrn; FcRn
UniProt Entry Name
FCGRN_MOUSE
Uniprot Description
FCGRT: Binds to the Fc region of monomeric immunoglobulins gamma. Mediates the uptake of IgG from milk. Possible role in transfer of immunoglobulin G from mother to fetus. Belongs to the immunoglobulin superfamily.
Protein type: Membrane protein, integral; Receptor, misc.; Immunoglobulin superfamily
Cellular Component: plasma membrane
Molecular Function: antigen binding; beta-2-microglobulin binding; IgG binding; IgG receptor activity
Biological Process: antigen processing and presentation
Research Articles on FCGRT
Similar Products
Product Notes
The Mouse FCGRT fcgrt (Catalog #AAA178527) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178527 ELISA Kit recognizes Mouse FCGRT. It is sometimes possible for the material contained within the vial of "FCRN/FCGRT, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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