Mouse Neutrophil Elastase ELISA Kit | ELANE elisa kit

Mouse Neutrophil Elastase ELISA Kit

Cat. Num. AAA8248432

• Gene Names: Elane; NE; Ela2; F430011M15Rik
• Reactivity: Mouse
• Synonyms: Neutrophil Elastase; Mouse Neutrophil Elastase ELISA Kit; ELA2; Neutrophil elastase; Bone marrow serine protease; Elastase-2; Human leukocyte elastase; HLE; Medullasin; PMN elastase; ELANE elisa kit

• Reactivity: Mouse
• Specificity: The Mouse Neutrophil Elastase ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Neutrophil Elastase proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 265

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ELANE elisa kit

Principle of the Assay: The Mouse Neutrophil Elastase ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Neutrophil Elastase in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse Neutrophil Elastase coated on a 96-well plate. Standards and samples are pipetted into the wells and Neutrophil Elastase present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Neutrophil Elastase antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neutrophil Elastase bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Neutrophil elastase is a serine protease of neutrophil and monocyte granules. Its key physiologic role is in innate host defense, but it can also participate in tissue remodeling and possesses secretagogue actions important to local inflammatory responses. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19.

NCBI and Uniprot Product Information

• NCBI GI #: 114052444
• NCBI GeneID: 50701
• NCBI Accession #: NP_056594.2
• NCBI GenBank Nucleotide #: NM_015779.2
• UniProt Accession #: Q3UP87; Q61515; A6H698
• Molecular Weight: 28,648 Da
• NCBI Official Full Name: neutrophil elastase
• NCBI Official Synonym Full Names: elastase, neutrophil expressed
• NCBI Official Symbol: Elane
• NCBI Official Synonym Symbols: NE; Ela2; F430011M15Rik
• NCBI Protein Information: neutrophil elastase
• UniProt Protein Name: Neutrophil elastase
• Protein Family: Neutrophil elastase
• UniProt Gene Name: Elane
• UniProt Synonym Gene Names: Ela2
• UniProt Entry Name: ELNE_MOUSE

NCBI Description

This gene encodes a member of the chymotrypsin-like family of serine protease enzymes that hydrolyzes a broad range of protein substrates including elastin. This gene is expressed by neutrophils where the encoded enzyme is stored in azurophil granules. Upon neutrophil activation, the active enzyme is released into the extracellular mileu. Mice lacking the encoded protein exhibit increased susceptibility to sepsis and death following intraperitoneal infection with Gram negative bacteria. This gene is located adjacent to a related proteinase gene on chromosome 10. [provided by RefSeq, Jul 2016]

Uniprot Description

ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily.

Protein type: Cell cycle regulation; Cell surface; EC 3.4.21.37; Motility/polarity/chemotaxis; Protease

Cellular Component: cell surface; cytoplasm; extracellular space; secretory granule; transcriptional repressor complex

Molecular Function: cytokine binding; endopeptidase activity; heparin binding; peptidase activity; protease binding; serine-type endopeptidase activity

Biological Process: acute inflammatory response to antigenic stimulus; defense response to bacterium; defense response to fungus; leukocyte migration; leukocyte migration during inflammatory response; negative regulation of chemokine biosynthetic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; phagocytosis; positive regulation of immune response; positive regulation of interleukin-8 biosynthetic process; positive regulation of smooth muscle cell proliferation; proteolysis; response to lipopolysaccharide; response to UV; response to yeast