Mouse Elastase 2, Neutrophil (ELA2) RTU ELISA Kit | ELA2 rtu elisa kit

Mouse Elastase 2, Neutrophil (ELA2) ELISA Kit

Cat. Num. AAA459032

• Gene Names: Elane; NE; Ela2; F430011M15Rik
• Reactivity: Mouse
• Synonyms: Elastase 2; Neutrophil (ELA2); Mouse Elastase 2; Neutrophil (ELA2) ELISA Kit; ELANE; HLE; HNE; NE; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Human Leukocyte Elastase; PMN Elastase; ELA2 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 265

• Assay Type: Sandwich
• Samples: Serum, Plasma or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.063ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ELA2 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ELA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ELA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ELA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 114052444
• NCBI GeneID: 50701
• NCBI Accession #: NP_056594.2
• NCBI GenBank Nucleotide #: NM_015779.2
• UniProt Accession #: Q3UP87; Q61515; A6H698
• Molecular Weight: 28,648 Da
• NCBI Official Full Name: neutrophil elastase
• NCBI Official Synonym Full Names: elastase, neutrophil expressed
• NCBI Official Symbol: Elane
• NCBI Official Synonym Symbols: NE; Ela2; F430011M15Rik
• NCBI Protein Information: neutrophil elastase
• UniProt Protein Name: Neutrophil elastase
• UniProt Gene Name: Elane
• UniProt Synonym Gene Names: Ela2

NCBI Description

This gene encodes a member of the chymotrypsin-like family of serine protease enzymes that hydrolyzes a broad range of protein substrates including elastin. This gene is expressed by neutrophils where the encoded enzyme is stored in azurophil granules. Upon neutrophil activation, the active enzyme is released into the extracellular mileu. Mice lacking the encoded protein exhibit increased susceptibility to sepsis and death following intraperitoneal infection with Gram negative bacteria. This gene is located adjacent to a related proteinase gene on chromosome 10. [provided by RefSeq, Jul 2016]

Uniprot Description

Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis ().