Human Persephin ELISA Kit | PSPN elisa kit

Human Persephin ELISA Kit

Cat. Num. AAA8248389

• Gene Names: PSPN; PSP
• Reactivity: Human
• Synonyms: Persephin; Human Persephin ELISA Kit; PSP; PSPN elisa kit

• Reactivity: Human
• Specificity: The Human Persephin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Persephin proteins within the range of 93.7 pg/ml - 6000 pg/ml.
• Sequence Length: 156

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSPN elisa kit

Principle of the Assay: The Human Persephin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Persephin in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human Persephin coated on a 96-well plate. Standards and samples are pipetted into the wells and Persephin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Persephin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Persephin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Persephin, also known as PSPN, is a neurotrophic factor in the glial cell line-derived neurotrophic factor (GDNF) family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. This PSPN gene is mapped to chromosome 19p13.3. The encoded protein has amino acid similarity to its other family members, glial cell line-derived neurotrophic factor and neurturin. This gene product promotes the survival of ventral midbrain dopaminergic neurons in culture and prevents their degeneration after 6-hydroxydopamine treatment in vivo.

NCBI and Uniprot Product Information

• NCBI GI #: 4758974
• NCBI GeneID: 5623
• NCBI Accession #: NP_004149.1
• NCBI GenBank Nucleotide #: NM_004158.3
• UniProt Accession #: O60542
• Molecular Weight: 16,600 Da
• NCBI Official Full Name: persephin preproprotein
• NCBI Official Synonym Full Names: persephin
• NCBI Official Symbol: PSPN
• NCBI Official Synonym Symbols: PSP
• NCBI Protein Information: persephin
• UniProt Protein Name: Persephin
• Protein Family: Persephin
• UniProt Gene Name: PSPN
• UniProt Synonym Gene Names: PSP
• UniProt Entry Name: PSPN_HUMAN

NCBI Description

This gene encodes a secreted ligand of the GDNF (glial cell line-derived neurotrophic factor) subfamily and TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein signals through the RET receptor tyrosine kinase and a GPI-linked coreceptor, and promotes survival of neuronal populations. This protein may play a role in cell death, and nervous system development and function. Elevated expression of this gene has been observed in oral squamous cell carcinoma. [provided by RefSeq, Aug 2016]

Uniprot Description

PSPN: Exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons. Belongs to the TGF-beta family. GDNF subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular region

Molecular Function: Ras guanyl-nucleotide exchange factor activity; receptor binding

Biological Process: central nervous system development; MAPKKK cascade; nervous system development