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Western Blot (WB) (Western blot analysis of MIA2 expression in HepG2 (A), HuvEc (B) whole cell lysates.)

Rabbit anti-Human MIA2 Polyclonal Antibody | anti-MIA2 antibody

Anti-MIA2 Antibody

Gene Names
MIA2; MEA6; MGEA; MGEA6; CTAGE5; MGEA11
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by affinity chromatography.
Synonyms
MIA2; Polyclonal Antibody; Anti-MIA2 Antibody; Melanoma inhibitory activity protein 2; anti-MIA2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of MIA2 protein.
Purity/Purification
The antibody was purified by affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
654
Applicable Applications for anti-MIA2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1/500 - 1/2000; Immunohistochemistry: 1/50 - 1/200
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MIA2. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of MIA2 expression in HepG2 (A), HuvEc (B) whole cell lysates.)

Western Blot (WB) (Western blot analysis of MIA2 expression in HepG2 (A), HuvEc (B) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of MIA2 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of MIA2 staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-MIA2 antibody
Rabbit polyclonal antibody to MIA2

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
74,089 Da
NCBI Official Full Name
melanoma inhibitory activity protein 2 isoform 9
NCBI Official Synonym Full Names
melanoma inhibitory activity 2
NCBI Official Symbol
MIA2
NCBI Official Synonym Symbols
MEA6; MGEA; MGEA6; CTAGE5; MGEA11
NCBI Protein Information
cTAGE family member 5; melanoma inhibitory activity protein 2
UniProt Protein Name
Melanoma inhibitory activity protein 2
UniProt Gene Name
MIA2
UniProt Entry Name
MIA2_HUMAN

NCBI Description

This gene encodes s receptor in the endoplasmic reticulum, which plays a role in the export of large pre-chylomicrons and pre-very low density lipoproteins (pre-VLDLs). Three major classes of transcripts are generated from this gene- melanoma inhibitory activity 2-specific transcripts, cTAGE family member 5-specific transcripts and transcripts that include exons from both these transcript species (TANGO1-like or TALI). Additionally, alternative splicing in these transcripts results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Sep 2016]

Uniprot Description

MIA2: May play a role in the pathophysiology of liver disease and may serve as a marker of liver damage. Belongs to the MIA/OTOR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q13.2

Research Articles on MIA2

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Product Notes

The MIA2 mia2 (Catalog #AAA8248270) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MIA2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/500 - 1/2000; Immunohistochemistry: 1/50 - 1/200. Researchers should empirically determine the suitability of the MIA2 mia2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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