Typical Testing Data/Standard Curve (for reference only)
Human Thioredoxin 1 ELISA Kit | TXN elisa kit
Human Thioredoxin 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: The Human Thioredoxin 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thioredoxin 1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Thioredoxin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Thioredoxin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Thioredoxin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thioredoxin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background: Sandwich Enzyme-Linked Immunosorbent Assay for Quantitative Detection of Human Thioredoxin 1 Concentrations in Cell Culture Supernatants, Serum, Plasma.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
TXN: Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3. In case of infection, interacts with S.typhimurium protein slrP. Interacts with APEX1; the interaction stimulates the FOS/JUN AP-1 DNA- binding activity in a redox-dependent manner. Up-regulated by ionizing radiation. Belongs to the thioredoxin family.
Protein type: Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 9q31
Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol; nucleus
Molecular Function: protein binding; protein disulfide oxidoreductase activity; peptide disulfide oxidoreductase activity
Biological Process: negative regulation of protein export from nucleus; transcription, DNA-dependent; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; activation of protein kinase B; negative regulation of transcription from RNA polymerase II promoter; signal transduction; positive regulation of peptidyl-serine phosphorylation; glycerol ether metabolic process; cell proliferation; positive regulation of protein kinase B signaling cascade; response to radiation; response to reactive oxygen species; cell-cell signaling; cell redox homeostasis; innate immune response; regulation of protein import into nucleus, translocation; cell motility; positive regulation of DNA binding
Research Articles on TXN
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Product Notes
The Human TXN txn (Catalog #AAA824785) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA824785 ELISA Kit recognizes Human TXN. AAA Biotech's Thioredoxin 1 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (SE). Researchers should empirically determine the suitability of the TXN txn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thioredoxin 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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