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Western Blot (WB) (Western blot analysis of RPLP0 expression in A549 (A), K562 (B), Jurkat (C), mouse liver (D), mouse testis (E) whole cell lysates.)

Rabbit RPLP0 Polyclonal Antibody | anti-RPLP0 antibody

Anti-RPLP0 Antibody

Gene Names
RPLP0; P0; LP0; L10E; RPP0; PRLP0
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
RPLP0; Polyclonal Antibody; Anti-RPLP0 Antibody; 60S acidic ribosomal protein P0; 60S ribosomal protein L10E; anti-RPLP0 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of RPLP0 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
317
Applicable Applications for anti-RPLP0 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
Western Blot: 1/500 - 1/2000, Immunofluorescence/Immunocytochemistry: 1/50 - 1/200
Immunogen
Recombinant full length protein of human RPLP0
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of RPLP0 expression in A549 (A), K562 (B), Jurkat (C), mouse liver (D), mouse testis (E) whole cell lysates.)

Western Blot (WB) (Western blot analysis of RPLP0 expression in A549 (A), K562 (B), Jurkat (C), mouse liver (D), mouse testis (E) whole cell lysates.)

Immunofluorescence (IF)

(Immunofluorescent analysis of RPLP0 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

Immunofluorescence (IF) (Immunofluorescent analysis of RPLP0 staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Related Product Information for anti-RPLP0 antibody
Rabbit polyclonal antibody to RPLP0

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,436 Da
NCBI Official Full Name
60S acidic ribosomal protein P0
NCBI Official Synonym Full Names
ribosomal protein lateral stalk subunit P0
NCBI Official Symbol
RPLP0
NCBI Official Synonym Symbols
P0; LP0; L10E; RPP0; PRLP0
NCBI Protein Information
60S acidic ribosomal protein P0
UniProt Protein Name
60S acidic ribosomal protein P0
UniProt Gene Name
RPLP0
UniProt Entry Name
RLA0_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPLP0: a ribosomal protein of the L10P family. Located in the cytoplasm and nucleus. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: mRNA catabolic process, nonsense-mediated decay; rRNA processing; SRP-dependent cotranslational protein targeting to membrane; translational initiation; viral transcription

Research Articles on RPLP0

Similar Products

Product Notes

The RPLP0 rplp0 (Catalog #AAA8247630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RPLP0 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RPLP0 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: 1/500 - 1/2000, Immunofluorescence/Immunocytochemistry: 1/50 - 1/200. Researchers should empirically determine the suitability of the RPLP0 rplp0 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RPLP0, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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