Human 60S acidic ribosomal protein P0 (RPLP0) ELISA Kit | RPLP0 elisa kit

Human 60S acidic ribosomal protein P0 (RPLP0) ELISA Kit

Cat. Num. AAA7203044

• Gene Names: RPLP0; P0; LP0; L10E; RPP0; PRLP0
• Reactivity: Human
• Synonyms: 60S acidic ribosomal protein P0 (RPLP0); Human 60S acidic ribosomal protein P0 (RPLP0) ELISA Kit; RPLP0 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RPLP0 elisa kit

Intended Uses: This RPLP0 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RPLP0.

Principle of the Assay: RPLP0 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPLP0 antibody and an RPLP0-HRP conjugate. The assay sample and buffer are incubated together with RPLP0-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPLP0 concentration since RPLP0 from samples and RPLP0-HRP conjugate compete for the anti-RPLP0 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPLP0 from the sample, fewer sites are left to bind RPLP0-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPLP0 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for RPLP0 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 16933546
• NCBI GeneID: 6175
• NCBI Accession #: NP_444505.1
• NCBI GenBank Nucleotide #: NM_053275.3
• Molecular Weight: 27,436 Da
• NCBI Official Full Name: 60S acidic ribosomal protein P0
• NCBI Official Synonym Full Names: ribosomal protein, large, P0
• NCBI Official Symbol: RPLP0
• NCBI Official Synonym Symbols: P0; LP0; L10E; RPP0; PRLP0
• NCBI Protein Information: 60S acidic ribosomal protein P0; 60S ribosomal protein L10E; acidic ribosomal phosphoprotein P0
• UniProt Protein Name: 60S acidic ribosomal protein P0
• UniProt Gene Name: RPLP0
• UniProt Entry Name: RLA0_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPLP0: a ribosomal protein of the L10P family. Located in the cytoplasm and nucleus. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: focal adhesion; membrane; cytoplasm; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; ribosome biogenesis and assembly; translational initiation; viral transcription; gene expression; translational termination; viral infectious cycle