Western Blot (WB)
(Western blot analysis of Rac 1 expression in BT474 (A), mouse liver (B), rat brain (C) whole cell lysates.)
Rabbit Rac 1 Polyclonal Antibody | anti-RAC1 antibody
Anti-Rac 1 Antibody
Western Blot (WB)
(Western blot analysis of Rac 1 expression in BT474 (A), mouse liver (B), rat brain (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Rac 1 expression in BT474 (A), mouse liver (B), rat brain (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Rac 1 staining in human gastric cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Rac 1 staining in human gastric cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Rac 1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of Rac 1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Uniprot Description
RAC1: a plasma membrane-associated member of the Rho-GTPase family. Plays a key role in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. GTP binding stimulates its activity. Phosphorylation by Akt may inhibit GTP binding of Rac1, therefore attenuating the downstream signal transduction pathway. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Two alternatively spliced isoforms have been described.
Protein type: G protein; G protein, monomeric; G protein, monomeric, Rho; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 7p22
Cellular Component: actin filament; cytoplasm; cytosol; early endosome membrane; endoplasmic reticulum membrane; extrinsic to plasma membrane; focal adhesion; Golgi membrane; lamellipodium; melanosome; membrane; nucleus; phagocytic cup; plasma membrane; trans-Golgi network
Molecular Function: enzyme binding; GTP binding; GTP-dependent protein binding; GTPase activity; histone deacetylase binding; protein binding; protein kinase binding; Rab GTPase binding; Rho GDP-dissociation inhibitor binding; thioesterase binding
Biological Process: actin cytoskeleton organization and biogenesis; actin filament polymerization; anatomical structure morphogenesis; auditory receptor cell morphogenesis; axon guidance; blood coagulation; bone resorption; cell adhesion; cell motility; cell motility involved in cell locomotion; cell proliferation; cell-matrix adhesion; cerebral cortex GABAergic interneuron development; cerebral cortex radially oriented cell migration; dendrite morphogenesis; embryonic olfactory bulb interneuron precursor migration; engulfment of apoptotic cell; ephrin receptor signaling pathway; G-protein coupled receptor protein signaling pathway; homeostasis of number of cells within a tissue; hyperosmotic response; inflammatory response; intercellular junction assembly and maintenance; lamellipodium biogenesis; localization within membrane; mast cell chemotaxis; metabolic process; negative regulation of interleukin-23 production; negative regulation of receptor-mediated endocytosis; organization of an anatomical structure; platelet activation; positive regulation of actin filament polymerization; positive regulation of apoptosis; positive regulation of DNA replication; positive regulation of focal adhesion formation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein amino acid phosphorylation; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; protein transport; regulation of cell migration; regulation of cell size; regulation of defense response to virus by virus; regulation of hydrogen peroxide metabolic process; regulation of neuron maturation; regulation of small GTPase mediated signal transduction; response to wounding; ruffle organization and biogenesis; small GTPase mediated signal transduction; synaptic transmission, GABAergic; T cell costimulation; vascular endothelial growth factor receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway
Research Articles on RAC1
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Product Notes
The RAC1 rac1 (Catalog #AAA8245759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rac 1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rac 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200; IF/ICC: 1/50 - 1/100. Researchers should empirically determine the suitability of the RAC1 rac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rac 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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