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Western Blot (WB) (Western blot analysis of PTPS expression in Hela (A), mouse liver (B), mouse heart (C), rat kindey (D) whole cell lysates.)

Rabbit PTPS Polyclonal Antibody | anti-PTS antibody

Anti-PTPS Antibody

Gene Names
PTS; PTPS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
PTPS; Polyclonal Antibody; Anti-PTPS Antibody; 6-pyruvoyl tetrahydrobiopterin synthase; PTP synthase; anti-PTS antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of PTPS protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
145
Applicable Applications for anti-PTS antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1/500 - 1/2000; IF/ICC: 1/10 - 1/100
Immunogen
Recombinant full length protein of human PTPS
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of PTPS expression in Hela (A), mouse liver (B), mouse heart (C), rat kindey (D) whole cell lysates.)

Western Blot (WB) (Western blot analysis of PTPS expression in Hela (A), mouse liver (B), mouse heart (C), rat kindey (D) whole cell lysates.)

Immunofluorescence (IF)

(Immunofluorescent analysis of PTPS staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

Immunofluorescence (IF) (Immunofluorescent analysis of PTPS staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Related Product Information for anti-PTS antibody
Rabbit polyclonal antibody to PTPS

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,386 Da
NCBI Official Full Name
6-pyruvoyl tetrahydrobiopterin synthase
NCBI Official Synonym Full Names
6-pyruvoyltetrahydropterin synthase
NCBI Official Symbol
PTS
NCBI Official Synonym Symbols
PTPS
NCBI Protein Information
6-pyruvoyl tetrahydrobiopterin synthase
UniProt Protein Name
6-pyruvoyl tetrahydrobiopterin synthase
UniProt Gene Name
PTS
UniProt Synonym Gene Names
PTP synthase; PTPS
UniProt Entry Name
PTPS_HUMAN

NCBI Description

The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH(4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. [provided by RefSeq, Oct 2008]

Uniprot Description

PTS: Involved in the biosynthesis of tetrahydrobiopterin, an essential cofactor of aromatic amino acid hydroxylases. Catalyzes the transformation of 7,8-dihydroneopterin triphosphate into 6- pyruvoyl tetrahydropterin. Defects in PTS are the cause of BH4-deficient hyperphenylalaninemia type A (HPABH4A); also called 6-pyruvoyl-tetrahydropterin synthase deficiency (PTS deficiency) or hyperphenylalaninemia tetrahydrobiopterin-deficient due to PTS deficiency. HPABH4A is an autosomal recessive disorder characterized by depletion of the neurotransmitters dopamine and serotonin, and clinically by severe neurological symptoms unresponsive to the classic phenylalanine-low diet. Belongs to the PTPS family.

Protein type: Mitochondrial; Cofactor and Vitamin Metabolism - folate biosynthesis; EC 4.2.3.12; Lyase

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: cytoplasm; cytosol; mitochondrion

Molecular Function: 6-pyruvoyltetrahydropterin synthase activity; identical protein binding; metal ion binding; protein binding; protein homodimerization activity

Biological Process: amino acid metabolic process; central nervous system development; regulation of nitric-oxide synthase activity; tetrahydrobiopterin biosynthetic process

Disease: Hyperphenylalaninemia, Bh4-deficient, A

Research Articles on PTS

Similar Products

Product Notes

The PTS pts (Catalog #AAA8245741) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PTPS Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PTPS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IF/ICC: 1/10 - 1/100. Researchers should empirically determine the suitability of the PTS pts for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTPS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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