General Monoamine Oxidase Assay Kit | Maoa assay kit

Monoamine Oxidase Microplate Assay Kit

Cat. Num. AAA8243176

• Gene Names: Maoa; Mao
• Reactivity: General
• Applications: Functional Assay
• Synonyms: Monoamine Oxidase; Monoamine Oxidase Microplate Assay Kit; EC 1.4.3.4; MAO; Monoamine oxidase; Adrenaline oxidase; Amine oxidase; Tyraminase; Tyramine oxidase; Maoa assay kit

• Reactivity: General
• Sequence Length: 526

• Applicable Applications for Maoa assay kit: Functional Assay
• Samples: Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media and Other biological fluids Samples.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months.

Related Product Information for Maoa assay kit

Background/Introduction: Monoamine oxidases (MAO, EC 1.4.3.4) are a family of enzymes that can oxidize a wide variety of endogenous primary amines. Two isoforms, MAO-A and MAO-B, have been identified based on their substrate, inhibitor specificity and tissue localization. Clonogenic studies have shown that these two isozymes have similar catalytic characteristics, yet their amino acid sequences are different. MAO-A favors Serotonin, Norerpinephrine and Dopamine as substrates, while phenylethylamine and benzylamine are MAO-B preferred substrates. MAO-A and MAO-B are mitochondrial-bound enzymes that are ubiquitously expressed throughout the brain and other tissues. Imbalance of MAOs levels has been associated with schizophrenia, depression, attention deficit and other disorders. MAO-A has been implicated in panic, anxiety and depression, whereas MAO-B defects result in Alzheimer's and Parkinson's diseases. The assay is initiated with the enzymatic catalysis of the substrate by MAO. The enzyme catalysed reaction products can be measured at a colorimetric readout at 360 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 220810
• NCBI GeneID: 29253
• Molecular Weight: 59,508 Da
• NCBI Official Full Name: monoamine oxidase, partial
• NCBI Official Synonym Full Names: monoamine oxidase A
• NCBI Official Symbol: Maoa
• NCBI Official Synonym Symbols: Mao
• NCBI Protein Information: amine oxidase [flavin-containing] A
• UniProt Protein Name: Amine oxidase [flavin-containing] A
• Protein Family: Monoamine oxidase
• UniProt Gene Name: Maoa
• UniProt Synonym Gene Names: MAO-A
• UniProt Entry Name: AOFA_RAT

NCBI Description

enzyme involved in the oxidative deamination of biogenic and xenobiotic amines [RGD, Feb 2006]

Uniprot Description

MAOA: Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine. Defects in MAOA are the cause of Brunner syndrome (BRUNS). Brunner syndrome is a form of X-linked non- dysmorphic mild mental retardation. Male patients are affected by a syndrome of borderline mental retardation and exhibit abnormal behavior, including disturbed regulation of impulsive aggression. Obligate female carriers have normal intelligence and behavior. Belongs to the flavin monoamine oxidase family.

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - histidine; EC 1.4.3.4; Membrane protein, integral; Oxidoreductase; Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - tyrosine

Cellular Component: integral to membrane; mitochondrial outer membrane; mitochondrion

Molecular Function: amine oxidase activity; FAD binding; serotonin binding

Biological Process: catecholamine catabolic process; dopamine catabolic process; neurotransmitter catabolic process; phenylethylamine metabolic process; serotonin metabolic process