MYO5A sirna

MYO5A siRNA (Mouse)

Cat. Num. AAA8241313

• Gene Names: Myo5a; d; Dbv; MVa; flr; Myo5; MyoVA; Sev-1; flail; d-120J; AI413174; AI661011; 9630007J19Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYO5A; MYO5A siRNA (Mouse); DILUTE; Unconventional myosin-Va; Dilute myosin heavy chain. non-muscle; MYO5A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: MYO5A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1853

• Applicable Applications for MYO5A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse MYO5A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYO5A sirna

siRNA to inhibit MYO5A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 115511052
• NCBI GeneID: 17918
• NCBI Accession #: NP_034994.2
• NCBI GenBank Nucleotide #: NM_010864.2
• UniProt Accession #: Q99104; E9PUE5
• Molecular Weight: 215,538 Da
• NCBI Official Full Name: unconventional myosin-Va
• NCBI Official Synonym Full Names: myosin VA
• NCBI Official Symbol: Myo5a
• NCBI Official Synonym Symbols: d; Dbv; MVa; flr; Myo5; MyoVA; Sev-1; flail; d-120J; AI413174; AI661011; 9630007J19Rik
• NCBI Protein Information: unconventional myosin-Va
• UniProt Protein Name: Unconventional myosin-Va
• Protein Family: Unconventional myosin
• UniProt Gene Name: Myo5a
• UniProt Synonym Gene Names: Dilute
• UniProt Entry Name: MYO5A_MOUSE

Uniprot Description

MYO5A: Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. May also be required for some polarization process involved in dendrite formation. May be a homodimer, which associates with multiple calmodulin or myosin light chains. Binds MLPH and MYRIP. Interacts with RIPL2, the interaction is required for its role in dendrite formation. Detected in melanocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis

Cellular Component: Golgi apparatus; filopodium tip; neuron projection; endoplasmic reticulum; lysosome; cell; early endosome; actomyosin; intermediate filament; actin filament; cytosol; secretory granule; ruffle; recycling endosome; synaptic vesicle; actomyosin, myosin complex part; photoreceptor outer segment; membrane; cell soma; axon; late endosome; cytoplasm; melanosome; peroxisome; myosin complex; vesicle

Molecular Function: protein dimerization activity; motor activity; nucleotide binding; calcium ion binding; actin binding; calcium-dependent protein binding; calmodulin binding; microfilament motor activity; ATP-dependent protein binding; protein binding; SNARE binding; protein heterodimerization activity; syntaxin-1 binding; ATP binding; Rab GTPase binding

Biological Process: myelination; exocytosis; melanin metabolic process; metabolic process; pigmentation during development; long-chain fatty acid biosynthetic process; locomotion during locomotory behavior; odontogenesis; synaptic transmission; vesicle transport along actin filament; protein transport; visual perception; transport; melanocyte differentiation; synapse organization and biogenesis; regulation of inositol-1,4,5-triphosphate receptor activity; neuromuscular process controlling balance; ER localization; actin filament-based movement; melanosome localization; melanosome transport; regulation of exocytosis; melanin biosynthetic process; vesicle-mediated transport; pigmentation; cellular response to insulin stimulus; insulin secretion; post-Golgi vesicle-mediated transport; anagen; secretory granule localization