AMICA1 sirna

AMICA1 siRNA (Human)

Cat. Num. AAA8241153

• Gene Names: AMICA1; JAML; AMICA; Gm638; CREA7-1; CREA7-4
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AMICA1; AMICA1 siRNA (Human); JAML; Junctional adhesion molecule-like; Adhesion molecule interacting with CXADR antigen 1; Dendritic cell-specific protein CREA7-1; AMICA1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: AMICA1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 355

• Applicable Applications for AMICA1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human AMICA1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AMICA1 sirna

siRNA to inhibit AMICA1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 148664207
• NCBI GeneID: 120425
• NCBI Accession #: NP_001091996.1
• NCBI GenBank Nucleotide #: NM_001098526.1
• UniProt Accession #: Q86YT9; Q496M1; Q5DTC6; Q7Z499; Q8N9I7; Q8NF70; B0YIV1; B0YIV2
• Molecular Weight: 40,136 Da
• NCBI Official Full Name: junctional adhesion molecule-like isoform 1
• NCBI Official Synonym Full Names: adhesion molecule, interacts with CXADR antigen 1
• NCBI Official Symbol: AMICA1
• NCBI Official Synonym Symbols: JAML; AMICA; Gm638; CREA7-1; CREA7-4
• NCBI Protein Information: junctional adhesion molecule-like
• UniProt Protein Name: Junctional adhesion molecule-like
• UniProt Gene Name: AMICA1
• UniProt Synonym Gene Names: JAML
• UniProt Entry Name: JAML1_HUMAN

Uniprot Description

AMICA1: an adhesion molecule that may function in the migration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, it mediates adhesive interactions with CXADR, a protein of the junctional complex of epithelial cells. Enhances myeloid leukemia cell adhesion to endothelial cells. Up-regulated upon retinoic acid, Me2SO and PMA treatment in differentiating myeloid leukemia cells. Three alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: tight junction; integral to membrane; plasma membrane

Molecular Function: integrin binding; protein homodimerization activity; cell adhesion molecule binding

Biological Process: heterophilic cell adhesion; neutrophil chemotaxis; regulation of immune response; positive regulation of epithelial cell proliferation involved in wound healing; blood coagulation; leukocyte migration; gamma-delta T cell activation