SCAMP5 sirna

SCAMP5 siRNA (Mouse)

Cat. Num. AAA8240862

• Gene Names: Scamp5; Sc5
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SCAMP5; SCAMP5 siRNA (Mouse); Secretory carrier-associated membrane protein 5; Secretory carrier membrane protein 5; SCAMP5 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: SCAMP5 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 235

• Applicable Applications for SCAMP5 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse SCAMP5 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SCAMP5 sirna

siRNA to inhibit SCAMP5 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 674781088
• NCBI GeneID: 56807
• NCBI Accession #: NP_001288563.1
• NCBI GenBank Nucleotide #: NM_001301634.1
• UniProt Accession #: Q9JKD3
• Molecular Weight: 26,068 Da
• NCBI Official Full Name: secretory carrier-associated membrane protein 5 isoform 1
• NCBI Official Synonym Full Names: secretory carrier membrane protein 5
• NCBI Official Symbol: Scamp5
• NCBI Official Synonym Symbols: Sc5
• NCBI Protein Information: secretory carrier-associated membrane protein 5
• UniProt Protein Name: Secretory carrier-associated membrane protein 5
• Protein Family: Secretory carrier-associated membrane protein
• UniProt Gene Name: Scamp5
• UniProt Synonym Gene Names: Secretory carrier membrane protein 5
• UniProt Entry Name: SCAM5_MOUSE

NCBI Description

This gene encodes a member of the Scamp (secretory carrier membrane protein) family. The encoded protein may be involved in neuronal vesicle trafficking. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]

Uniprot Description

SCAMP5: Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway. Belongs to the SCAMP family. SCAMP5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Cellular Component: Golgi apparatus; recycling endosome membrane; integral to membrane; Golgi membrane; SNARE complex; synaptic vesicle; membrane; plasma membrane; trans-Golgi network membrane; synapse; cytoplasmic vesicle; cell junction; endosome

Biological Process: positive regulation of calcium ion-dependent exocytosis; protein transport; exocytosis; transport; negative regulation of endocytosis; positive regulation of cytokine secretion