FZD4 sirna

FZD4 siRNA (Human)

Cat. Num. AAA8240333

• Gene Names: FZD4; Fz4; EVR1; FEVR; Fz-4; FzE4; GPCR; hFz4; CD344; FZD4S
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: FZD4; FZD4 siRNA (Human); Frizzled-4; Fz-4; hFz4; FzE4; CD antigen CD344; FZD4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: FZD4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 537

• Applicable Applications for FZD4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human FZD4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for FZD4 sirna

siRNA to inhibit FZD4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22547161
• NCBI GeneID: 8322
• NCBI Accession #: NP_036325.2
• NCBI GenBank Nucleotide #: NM_012193.3
• UniProt Accession #: Q9ULV1; Q14C97; Q6S9E4; A8K9Q3
• Molecular Weight: 59,881 Da
• NCBI Official Full Name: frizzled-4
• NCBI Official Synonym Full Names: frizzled class receptor 4
• NCBI Official Symbol: FZD4
• NCBI Official Synonym Symbols: Fz4; EVR1; FEVR; Fz-4; FzE4; GPCR; hFz4; CD344; FZD4S
• NCBI Protein Information: frizzled-4
• UniProt Protein Name: Frizzled-4
• UniProt Gene Name: FZD4
• UniProt Synonym Gene Names: Fz-4; hFz4
• UniProt Entry Name: FZD4_HUMAN

NCBI Description

This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This protein may play a role as a positive regulator of the Wingless type MMTV integration site signaling pathway. A transcript variant retaining intronic sequence and encoding a shorter isoform has been described, however, its expression is not supported by other experimental evidence. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD4: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Defects in FZD4 are the cause of vitreoretinopathy exudative type 1 (EVR1); also known as autosomal dominant familial exudative vitreoretinopathy (FEVR) or Criswick- Schepens syndrome. EVR1 is a disorder of the retinal vasculature characterized by an abrupt cessation of growth of peripheral capillaries, leading to an avascular peripheral retina. This may lead to compensatory retinal neovascularization, which is thought to be induced by hypoxia from the initial avascular insult. New vessels are prone to leakage and rupture causing exudates and bleeding, followed by scarring, retinal detachment and blindness. Clinical features can be highly variable, even within the same family. Patients with mild forms of the disease are asymptomatic, and their only disease-related abnormality is an arc of avascular retina in the extreme temporal periphery. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: GPCR, Fz/Smo family; Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 11q14.2

Cellular Component: cell surface; integral to plasma membrane; integral to membrane; plasma membrane; intercellular junction

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding; protein homodimerization activity; protein heterodimerization activity; cytokine binding; ubiquitin protein ligase binding; PDZ domain binding

Biological Process: neuron differentiation; positive regulation of JNK activity; G-protein coupled receptor protein signaling pathway; Wnt receptor signaling pathway, calcium modulating pathway; Wnt receptor signaling pathway; sensory perception of sound; positive regulation of transcription, DNA-dependent; regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of transcription factor activity; Wnt receptor signaling pathway through beta-catenin; vasculogenesis; progesterone secretion; locomotion during locomotory behavior

Disease: Exudative Vitreoretinopathy 1