AIM2 sirna

AIM2 siRNA (Mouse)

Cat. Num. AAA8240019

• Gene Names: Aim2; Gm1313; Ifi210
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AIM2; AIM2 siRNA (Mouse); GM1313; IFI210; Interferon-inducible protein AIM2; Interferon-inducible protein 210; Ifi-210; Interferon-inducible protein p210; AIM2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AIM2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 354

• Applicable Applications for AIM2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AIM2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AIM2 sirna

siRNA to inhibit AIM2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 162461985
• NCBI GeneID: 383619
• NCBI Accession #: NP_001013801.2
• NCBI GenBank Nucleotide #: NM_001013779.2
• UniProt Accession #: Q91VJ1
• Molecular Weight: 40,155 Da
• NCBI Official Full Name: interferon-inducible protein AIM2
• NCBI Official Synonym Full Names: absent in melanoma 2
• NCBI Official Symbol: Aim2
• NCBI Official Synonym Symbols: Gm1313; Ifi210
• NCBI Protein Information: interferon-inducible protein AIM2
• UniProt Protein Name: Interferon-inducible protein AIM2
• Protein Family: Protein
• UniProt Gene Name: Aim2
• UniProt Synonym Gene Names: Gm1313; Ifi210; Ifi-210
• UniProt Entry Name: AIM2_MOUSE

Uniprot Description

AIM2: a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity. Induced by interferon gamma. Defects in AIM2 may be a cause of microsatellite unstable colon cancers. AIM2 and various NOD-like receptors form multiprotein complexes called inflammasomes, which mediate caspase-1-dependent processing of pro-IL-1beta.

Protein type: Tumor suppressor

Cellular Component: cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; DNA binding; double-stranded DNA binding

Biological Process: positive regulation of interleukin-1 beta production; positive regulation of protein oligomerization; tumor necrosis factor-mediated signaling pathway; apoptosis; inhibition of NF-kappaB transcription factor; immune system process; innate immune response; interleukin-1 beta secretion; positive regulation of interleukin-1 beta secretion; inflammatory response; positive regulation of defense response to virus by host; activation of innate immune response; activation of NF-kappaB transcription factor