IRGM1 sirna

IRGM1 siRNA (Mouse)

Cat. Num. AAA8239522

• Gene Names: Irgm1; Ifi1; Irgm; Iigp3; Iipg3; Ifggd3; LRG-47
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: IRGM1; IRGM1 siRNA (Mouse); IFI1; IIGP3; IRGM; Immunity-related GTPase family M protein 1; Interferon-inducible GTPase 3; Interferon-inducible protein 1; LPS-stimulated RAW 264.7 macrophage protein 47; LRG-47; IRGM1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: IRGM1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 409

• Applicable Applications for IRGM1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse IRGM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for IRGM1 sirna

siRNA to inhibit IRGM1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6680351
• NCBI GeneID: 15944
• NCBI Accession #: NP_032352.1
• NCBI GenBank Nucleotide #: NM_008326.1
• UniProt Accession #: Q60766; Q3TJ73
• Molecular Weight: 44,888 Da
• NCBI Official Full Name: immunity-related GTPase family M protein 1
• NCBI Official Synonym Full Names: immunity-related GTPase family M member 1
• NCBI Official Symbol: Irgm1
• NCBI Official Synonym Symbols: Ifi1; Irgm; Iigp3; Iipg3; Ifggd3; LRG-47
• NCBI Protein Information: immunity-related GTPase family M protein 1
• UniProt Protein Name: Immunity-related GTPase family M protein 1
• Protein Family: Immunity-related GTPase family M protein
• UniProt Gene Name: Irgm1
• UniProt Synonym Gene Names: Ifi1; Iigp3; Irgm; LRG-47
• UniProt Entry Name: IRGM1_MOUSE

Uniprot Description

IRGM: Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility. Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19). A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Belongs to the interferon-inducible GTPase family.

Protein type: EC 3.6.5.-; Endoplasmic reticulum; Hydrolase

Cellular Component: Golgi apparatus; cell projection; membrane; endoplasmic reticulum; lysosome; late endosome; plasma membrane; cytoplasmic vesicle; endosome

Molecular Function: GTP binding; hydrolase activity; nucleotide binding

Biological Process: immune system process; innate immune response; autophagy; defense response