LOXL1 sirna

LOXL1 siRNA (Human)

Cat. Num. AAA8239450

• Gene Names: LOXL1; LOL; LOXL
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: LOXL1; LOXL1 siRNA (Human); LOXL; Lysyl oxidase homolog 1; Lysyl oxidase-like protein 1; LOL; LOXL1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: LOXL1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 574

• Applicable Applications for LOXL1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human LOXL1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for LOXL1 sirna

siRNA to inhibit LOXL1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 67782346
• NCBI GeneID: 4016
• NCBI Accession #: NP_005567.2
• NCBI GenBank Nucleotide #: NM_005576.2
• UniProt Accession #: Q08397; Q6NUL3; Q96BW7
• Molecular Weight: 63,110 Da
• NCBI Official Full Name: lysyl oxidase homolog 1 preproprotein
• NCBI Official Synonym Full Names: lysyl oxidase-like 1
• NCBI Official Symbol: LOXL1
• NCBI Official Synonym Symbols: LOL; LOXL
• NCBI Protein Information: lysyl oxidase homolog 1
• UniProt Protein Name: Lysyl oxidase homolog 1
• Protein Family: Lysyl oxidase
• UniProt Gene Name: LOXL1
• UniProt Synonym Gene Names: LOXL; LOL
• UniProt Entry Name: LOXL1_HUMAN

NCBI Description

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]

Uniprot Description

LOXL1: Active on elastin and collagen substrates. Genetic variations in LOXL1 are a cause of susceptibility to exfoliation syndrome (XFS); also called exfoliation glaucoma (XFG). XFS is a disorder characterized by accumulation of abnormal fibrillar deposits in the anterior segment of the eye. In addition to being a cause of glaucoma and glaucomatous optic neuropathy, exfoliation syndrome has also been associated with lens zonule weakness, cataract formation, and systemic vascular complications due to deposition of exfoliation material in extraocular tissues. Susceptibility to exfoliation syndrome is conferred by a risk haplotype that includes two LOXL1 coding non-synonymous SNPs (Arg141Leu and Gly153Asp) and one intronic SNP. Arg141Leu and Gly153Asp are sufficient to confer disease susceptibility in some populations. Belongs to the lysyl oxidase family.

Protein type: EC 1.4.3.-; Secreted, signal peptide; Secreted; Extracellular matrix; Oxidoreductase

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: extracellular matrix; extracellular space; acrosome; extracellular region; basement membrane

Molecular Function: copper ion binding; oxidoreductase activity, acting on the CH-NH2 group of donors, oxygen as acceptor

Biological Process: extracellular matrix organization and biogenesis; response to lipopolysaccharide; protein amino acid deamination

Disease: Exfoliation Syndrome