ATF2 sirna

ATF2 siRNA (Rat)

Cat. Num. AAA8239182

• Reactivity: Rat
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATF2; ATF2 siRNA (Rat); Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; cAMP response element-binding protein CRE-BP1; ATF2 sirna

• Host: Synthetic
• Reactivity: Rat
• Specificity: ATF2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 487

• Applicable Applications for ATF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ATF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATF2 sirna

siRNA to inhibit ATF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13591926
• NCBI GeneID: 81647
• NCBI Accession #: NP_112280.1
• NCBI GenBank Nucleotide #: NM_031018.1
• UniProt Accession #: Q00969; Q62870
• Molecular Weight: 42,320 Da
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-2
• NCBI Official Synonym Full Names: activating transcription factor 2
• NCBI Official Symbol: Atf2
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-2
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-2
• Protein Family: Alcohol O-acetyltransferase
• UniProt Gene Name: Atf2
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-2
• UniProt Entry Name: ATF2_RAT

NCBI Description

transactivates tyrosine hydroxylase transcription; may be involved in regulation of catecholamine synthesis during neural development [RGD, Feb 2006]

Uniprot Description

ATF-2: a transcription factor that is a member of the leucine zipper family. Binds to the cAMP-responsive element (CRE). Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Also possesses histone acetyltransferase (HAT) activity that specifically acetylates histones H2B and H4 in vitro.

Protein type: Transcription factor; EC 2.3.1.48; C2H2-type zinc finger protein

Cellular Component: nucleoplasm; mitochondrial outer membrane; membrane; cytoplasm; nucleus

Molecular Function: histone acetyltransferase activity; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; metal ion binding; cAMP response element binding protein binding; chromatin binding; protein kinase binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; intra-S DNA damage checkpoint; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; histone acetylation; response to osmotic stress; positive regulation of transforming growth factor-beta2 production; response to DNA damage stimulus