SPINK1 sirna

SPINK1 siRNA (Human)

Cat. Num. AAA8238057

• Gene Names: SPINK1; TCP; PCTT; PSTI; TATI; Spink3
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: SPINK1; SPINK1 siRNA (Human); PSTI; Pancreatic secretory trypsin inhibitor; Serine protease inhibitor Kazal-type 1; Tumor-associated trypsin inhibitor; TATI; SPINK1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: SPINK1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 79

• Applicable Applications for SPINK1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human SPINK1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for SPINK1 sirna

siRNA to inhibit SPINK1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 45505132
• NCBI GeneID: 6690
• NCBI Accession #: NP_003113.2
• NCBI GenBank Nucleotide #: NM_003122.4
• UniProt Accession #: P00995
• Molecular Weight: 8,507 Da
• NCBI Official Full Name: pancreatic secretory trypsin inhibitor
• NCBI Official Synonym Full Names: serine peptidase inhibitor, Kazal type 1
• NCBI Official Symbol: SPINK1
• NCBI Official Synonym Symbols: TCP; PCTT; PSTI; TATI; Spink3
• NCBI Protein Information: pancreatic secretory trypsin inhibitor
• UniProt Protein Name: Pancreatic secretory trypsin inhibitor
• Protein Family: Serine protease inhibitor
• UniProt Gene Name: SPINK1
• UniProt Synonym Gene Names: PSTI; TATI
• UniProt Entry Name: ISK1_HUMAN

NCBI Description

The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq, Oct 2008]

Uniprot Description

SPINK1: This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas. Defects in SPINK1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Defects in SPINK1 are the cause of susceptibility to tropical calcific pancreatitis (TCP). TCP is an idiopathic, juvenile, nonalcoholic form of chronic pancreatitis widely prevalent in several tropical countries. It can be associated with fibrocalculous pancreatic diabetes (FCPD) depending on both environmental and genetic factors. TCP differs from alcoholic pancreatitis by a much younger age of onset, pancreatic calcification, a high incidence of insulin dependent but ketosis resistant diabetes mellitus, and an exceptionally high incidence of pancreatic cancer.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; endopeptidase inhibitor activity

Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; regulation of acrosome reaction

Disease: Tropical Calcific Pancreatitis; Pancreatitis, Hereditary