MYL4 sirna

MYL4 siRNA (Human)

Cat. Num. AAA8238051

• Gene Names: MYL4; GT1; ALC1; AMLC; PRO1957
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: MYL4; MYL4 siRNA (Human); MLC1; Myosin light chain 4; Myosin light chain 1. embryonic muscle/atrial isoform; Myosin light chain alkali GT-1 isoform; MYL4 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: MYL4 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 197

• Applicable Applications for MYL4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human MYL4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for MYL4 sirna

siRNA to inhibit MYL4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 50845428
• NCBI GeneID: 4635
• NCBI Accession #: NP_001002841.1
• NCBI GenBank Nucleotide #: NM_001002841.1
• UniProt Accession #: P12829; P11783; D3DXJ7
• Molecular Weight: 21,565 Da
• NCBI Official Full Name: myosin light chain 4
• NCBI Official Synonym Full Names: myosin, light chain 4, alkali; atrial, embryonic
• NCBI Official Symbol: MYL4
• NCBI Official Synonym Symbols: GT1; ALC1; AMLC; PRO1957
• NCBI Protein Information: myosin light chain 4
• UniProt Protein Name: Myosin light chain 4
• Protein Family: Myosin
• UniProt Gene Name: MYL4
• UniProt Synonym Gene Names: MLC1
• UniProt Entry Name: MYL4_HUMAN

NCBI Description

Myosin is a hexameric ATPase cellular motor protein. It is composed of two myosin heavy chains, two nonphosphorylatable myosin alkali light chains, and two phosphorylatable myosin regulatory light chains. This gene encodes a myosin alkali light chain that is found in embryonic muscle and adult atria. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MYL4: Regulatory light chain of myosin. Does not bind calcium.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Contractile

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cytosol; myosin complex; A band

Molecular Function: actin monomer binding; actin filament binding; myosin II heavy chain binding; calcium ion binding

Biological Process: positive regulation of ATPase activity; regulation of the force of heart contraction; cardiac muscle contraction; muscle filament sliding